摘要
乙烯利可诱导玉米根皮层细胞发生死亡解体而形成通气组织.随着乙烯利浓度的升高,根皮层细胞的死亡数目升高.利用TUNEL(末端标记法)实验显示:根皮层细胞核DNA发生了断裂现象,出现了暴露的3′OH末端.这为乙烯利诱导的通气组织形成中有程序化细胞死亡(PCO)的发生首次提供了直接证据;同时在外源乙烯利处理的玉米根部发现SOD和CAT活性显著下降,继而造成活性氧的积累,由于活性氧是诱导PCD的重要原因,因此,外源乙烯利诱发的PCD的发生极有可能是通过活性氧介导的.
Ethylene can induce the deach of cortex cells of Maize root and then induce the formation of Aerenchyma. With the increment of the concentration of ethylene, the number of death cells increases. Using TUNEL (terminal deoxynculeotidyl transferasemediated dUTP nick end labeling) experiment, it is found that nDNA of cortex cell is fragmented and the number of 3′OH terminals of DNA is increased. These results firstly provide directl evidence that ethylene induced formation of Aerechyma involves PCD. At the same time, it was f ound that the activity of SOD and CAT in the root of reactive oxygen species (ROS) decrease clearly . Since ROS can induce PCD, the PCD induced by the extraneous ethelene maybe mediated by ROS.
出处
《华中师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2000年第4期463-467,共5页
Journal of Central China Normal University：Natural Sciences
基金
国家重点基础研究专项经费资助!(G1999011700)
