摘要
应用硅胶、ODS、大孔树脂、葡聚糖凝胶Sephadex LH-20和HPLC等多种色谱分离方法相结合,从中药山慈菇来源之一——独蒜兰干燥假鳞茎的95%乙醇提取物的醋酸乙酯萃取部位中分离得到14个化合物,其中菲及二氢菲类10个,联苄类2个,木脂素1个,甾醇1个;根据化合物的波谱数据鉴定化合物的结构为monbarbatain A(1),2,7,2'-三羟基-4,4',7'-三甲氧基-1,1'-二聚菲(2),白芨烯A(3),pleionesin B(4),shanciol H(5),{7-hydroxy-7'-(4'-hydroxy-3'-methoxyphenyl)-4-methoxy-9,10,7',8'-tetrahydrophenanthro[2,3-b]furan-8'-yl}methyl acetate(6),2,7-二羟基-1-(对羟基苄基)-4-甲氧基菲(7),2,7-二羟基-1-(对-羟基苄基)-4-甲氧基-9,10-二氢菲(8),赫尔西酚(9),贝母兰宁(10),石斛酚(11),山药素Ⅲ(12),丁香脂素(13),麦角甾-4,6,8(14),22-四烯-3-酮(14)。其中化合物1-3,9,13,14为首次从该属植物中分离得到。
Fourteen compoumds were isolated from the ethyl acetate portion of the 95% ethanolic extract of Pleione bulbocodioides by a combination of various chromatographic techniques including silica gel, ODS, macroporous adsorbent resin, Sephadex LH- 20, and preparative HPLC, of which ten compoumds were phenanthrenes and dihydrophenanthrenes, two compoumds were bibenzyls, one was lignan and a sterol. Their structures were identified on the basis of spectroscopic data as monbarbatain A（1）, 2, 7, 2＇-trihy- droxy-4, 4＇, 7＇-trimethoxy-1, 1＇- biphenanthrene（2）, blestriarene A（3）, pleionesin B（4）, shaneiol H（$）, /7-hydroxy-7＇-（4＇-hy- droxy-3＇-methoxyphenyl） - 4 -methoxy-9, 10, 7＇, 8 ＇-tetrahydrophenanthro [ 2, 3-b ] furan-8＇-yl } methyl acetate （ 6 ）, 1 -p -hydroxybenzyl - 4-methoxy phenanthrene-2, 7-diol （7）, 1-p-hydroxybenzyl-4-met-hoxy-9, 10-dihydrophenanthrene-2, 7-diol （ 8 ）, hircinol （ 9 ）, coelonin（10）, gigantol（ll）, batatasin 11I（12）, syringaresinol（13） and ergosta-4, 6, 8（14）, 22-tetraen-3-one（14）. Compounds 1-3, 9, 13 and 14 were isolated from this genus for the first time.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2014年第3期442-447,共6页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(811733529)
关键词
山慈菇
独蒜兰
菲及二氢菲类
联苄类
Pseudobulbus Cremastrae seu Pleiones
Pleione bulbocodioides
phenanthrene and dihydrophenanthrene
bibenzyl
