摘要
采用超声辅助技术从香蕉皮中提取多酚类物质,利用响应曲面法建立多酚提取率与液料比、乙醇体积分数、超声温度、超声时间之间的数学模型,确定香蕉皮多酚的适宜提取工艺参数;通过体外实验评价香蕉皮多酚的抗氧化能力。结果表明:多酚提取率模型拟合度良好,超声辅助提取香蕉皮多酚的适宜工艺参数为液料比9∶1(mL/g)、乙醇体积分数54%、超声温度53℃、超声时间43 min,在此条件下多酚提取率为2.931%,提取的香蕉皮多酚具有较强的清除DPPH自由基、超氧阴离子自由基和羟自由基的能力,半抑制质量浓度分别为0.422 8、0.255 2 mg/mL和0.243 9 mg/mL。
Response surface methodology （RSM） was employed to optimize liquid-to-material ratio, ethanol concentration, ultrasound radiation time and temperature to obtain a high yield of polyphenols from banana peel by an ultrasonic-assisted extraction technique. The antioxidant activities of polyphenols extracted from banana peel were evaluated by in vitro assays. The results showed that the RSM model developed was able to describe the relationship between the factors （extraction parameters） and the response variable （polyphenol yield）. The optimized conditions were determined as follows： solvent- to-solid ratio, 9：1 （mL/g）; ethanol concentration, 54%; temperature, 53 ℃; and ultrasound radiation time, 43 rain. Under these conditions, the polyphenol yield obtained was 2.931%. The resulting extract exhibited significant 2,2-diphenyl- 1-picrylhydrazyl （DPPH）, superoxide anion and hydroxyl radical scavenging activities, and the concentrations for 50% inhibition （ICs0） were 0.422 8, 0.255 2 and 0.243 9 mg/mL, respectively.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2014年第2期12-17,共6页
Food Science
基金
“十二五”国家科技支撑计划项目(2012BAD38B05)
国家自然科学基金面上项目(31172009)
大连市科技计划项目(2012E13SF106)
大连民族学院博士启动基金项目(0701-110014)
关键词
香蕉皮
多酚
超声辅助提取
响应曲面
抗氧化
banana peel
polyphenol
ultrasound assisted extraction
response surface methodology
antioxidant activity
