摘要
试验从传统乳制品、仔猪粪便及鸡肠道分离到6株乳酸菌,分别命名为LS、LT、LJ1、LJ2、LZ1、LZ2。通过细菌形态学、生理生化特性和16SrDNA序列同源性分析,对6株分离菌进行鉴定及研究。结果显示LS、LT、LJ1、LZ2菌株为短乳杆菌;LJ2菌株为粪肠球菌;LZ1菌株为嗜酸乳杆菌。这6株菌株对李斯特氏菌、沙门氏菌、大肠杆菌和金黄色葡萄球菌均有明显的抑菌效果。
Six strains of Lactobacillus were isolated from traditional indigenous dairy products, piglet＇s feces and chicken intestine, designated as LS, LT, LJ1, LJ2, LZ1 and LZ2, respectively. Identifications were done according to bacteria mor- phology, physiological and biochemical properties, 16S rDNA sequence homology analysis, and their characteristics were re- searched. The results showed that LS, LT, LJ1 and LZ2 strains were Lactobacillus brevis, LJ2 strain was Enterococcus faeci- urn, LZ1 strain was Lactobacillus acidophilus. All of the six strains had obvious bacteriostasis effect on Listeria rnonocyto- genes, Salmonella typhirnuriurn , Escherichia coli and Staphylococcus aureus.
出处
《中国畜牧兽医》
CAS
北大核心
2014年第2期208-212,共5页
China Animal Husbandry & Veterinary Medicine
基金
国家自然基金(31160508)
关键词
乳酸菌
分离鉴定
16S
RDNA
Lactobacillus
isolation and identification
16S rDNA
