摘要
为探讨钼酸铵比色法测定大米中过氧化氢酶活性与粮油检验现行国家标准中的高锰酸钾滴定法的差异,选择6个不同产地大米用2种方法测定进行了多组测定比较。测定数据分析表明,6个产地样本过氧化氢酶活动度(mgH2O2/g)最低值组8.05,最高值组13.88。钼酸铵法测定的酶活动度总体平均值为11.66,略高于高锰酸钾滴定法的酶活动度总体平均值10.22。从测定方法的稳定性方面,高锰酸钾法测定6组内平均差值为5.78,钼酸铵法为3.44,钼酸铵法稳定性明显好于高锰酸钾法,更能反映不同样本间的差异。6个产地的大米中,偏北方产地样品过氧化氢酶活动度略高于偏南方产地样品,2种方法测定值呈现极显著相关性(2批测定分别为r=0.706**;r=0.702**P<0.01)。
To compare the difference between ammonium molybdate colorimetric method and potassium perman- ganate titration method （ GB/T 5522--2008） on the determination of rice catalase activity, six kinds of different ori- gin rice have been measured by these two methods. The results showed that the catalase activity （mgH2 O2/g） in six samples changed between 8.05 to 13.88, and the enzyme activity measured by ammonium molybdate method had overall average of 11.66, slightly higher than overall average of 10.22 which had measured by potassium per- manganate titration. From the aspect of determination method of stability, potassium permanganate method within the six group average difference was 5.78, ammonium molybdate method of 3.44, Ammonium molybdate method has sig- nificant benefits for reflecting the differences between different samples according to the compare results, and it was more suitable for the inspection of grains and oilseeds. The catalase activity in partial northern regions samples were slightly higher than the south regions samples in the six regions of rice tested. The two methods measurements showed si^ificant correlation （r = 0.706 ＊ ＊ ：r =0.702 ＊ ＊ .P〈0.01）.
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
2014年第2期89-93,99,共6页
Journal of the Chinese Cereals and Oils Association
关键词
大米
过氧化氢酶
钼酸铵比色法
高锰酸钾滴定法
rice, catalase, ammonium molybdate spectrophotometry, potassium permanganate titration
