摘要
采用多重培养基分离培养,结合16SrDNA鉴定以及PCR-变性凝胶梯度电泳(PCR-DGGE),对新鲜和冰鲜贮藏6 d的大黄鱼肠腔细菌类型和菌群结构进行鉴定。大黄鱼肠腔细菌有希瓦氏菌、假单胞菌、气单胞菌、普罗威登斯菌、摩根氏菌、环丝菌、嗜冷菌、不动杆菌、大肠杆菌、屎肠球菌、硬壁菌门的成员,其中希瓦氏菌和假单胞菌是鱼肠腔的优势细菌和重要的致腐细菌。PCR-DGGE图谱主成分分析(PCA)显示,假单胞菌是该菌群的特征菌落,冰鲜贮藏后期希瓦氏菌比例显著上升。多重培养基分离检测和PCR-DGGE都可检测出鱼肠道中普遍存在的细菌类群。PCR-DGGE培养基具有快速、较全面分析菌群结构的优点,可作为冰鲜鱼质量安全检测的方法。
The bacterial species composition and distribution in the intestines of large yellow croaker（Pseudosciaena crocea） during iced storage were investigated by both culture-dependent method and culture-independent method of poly-merase chain reaction and denaturing gradient gel electrophoresis（PCR-DGGE）.Eleven different bacterial species were i-dentified from spoiage fish samples,including Shewanella,Pseudomonas,Aeromonas,Providencia,Morganella,Bro-chothrix,Acinetobacter,Enterococcus faecium,Escherichia coli,Firmicute and Psychrobacter.Shewanella and Pseu-domonas were dominant bacterial groups.The principal component analysis（PCA） of DGGE fingerprint showed that bacte-rial flora of flesh fish intestines was similar with spoilage one,and Pseudomonas was highly associated with the flora.The proportion of Shewanella increased in bacterial flora after storage obviously.The dominant bacterial speices could been identified by both culture-dependent and PCR-DGGE methods.PCR-DGGE was used to investigate the bacterial diversity and population shifts among samples from different storage times more effectively.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2013年第12期188-196,共9页
Journal of Chinese Institute Of Food Science and Technology
