摘要
C-14-glutamate and C-14-arginine were spreaded on leaves of six-day old barley (Hordeum vulgare L.) seedlings that were treated with NaCl 200 mmol/L. The result showed that the pathway of arginine-->ornithine-->proline existed in the six-day old barley seedlings and was provoked remarkably by NaCl treatment. After seven days, proline accumulation contributed via the arginine-->ornithine-->proline pathway was 1.0 - 1.5 folds of that via the glutamate-->proline pathway. The activation of arginine-->ornithine-->proline pathway by salt stress in the salt-tolerant cultivar 'Jian 4' was 1.7 - 2.0 folds of that in the salt-sensitive cultivar 'KP 7', which suggested that the activation of arginine-->ornithine-->proline pathway in barley seedlings played an important role in improving salt tolerance of plants.
20 0mmol/LNaCl处理结合14 C_Glu和14 C_Arg叶面饲喂 6d龄大麦 (HordeumvulgareL .)幼苗 ,结果证明 6d龄大麦幼苗体内普遍存在Arg(谷氨酸 )→Orn(鸟氨酸 )→Pro(脯氨酸 )途径。盐胁迫明显激活了Arg→Orn→Pro途径 ,胁迫处理 7d ,大麦幼苗叶片和根系中该途径对Pro含量上升的贡献是Glu→Pro途径的 1.0～ 1.5倍。耐盐的“鉴 4”品种Arg→Orn→Pro途径对Pro含量上升的贡献是对盐敏感的“科品 7号”的 1.7～ 2 .0倍 。
基金
SupportedbytheNationalNaturalScienceFoundationofChina ( 3 0 0 70 45 5 ) .
