摘要
对遗传工程荧光假单胞菌IPP2 0 2进行了质粒稳定性检测、抑菌活性测定、在棉花根部和叶面定殖能力分析、杀虫蛋白抗紫外能力检测及田间杀虫活性测定等试验。结果表明 ,工程菌与出发菌株P30 3相比 ,其抑菌、定殖等有益于植物的优良特性未发生显著变化 ;经过连续培养和连续稀释培养后工程菌的质粒都非常稳定 ;广波长紫外线照射 2h后 ,苏云金芽胞杆菌 (Bacillusthuringiensis,简称Bt)由于裸露的伴孢晶体杀虫蛋白受紫外线破坏因而杀虫活性大大下降 ,而荧光假单胞菌工程菌的活性变化不大 ;IPP2 0 2田间杀虫活性与Bt野生菌株接近。工程菌有望解决Bt本身存在的杀虫蛋白多以裸露晶体的形式存在而易受紫外线破坏的弱点 ,同时也发挥了P30 3菌株在多种植物上定殖能力的优点 ,使其具有在植物周围大量繁殖而直到杀虫作用的优势。通过进一步研究 ,将有望构建成更有实用价值的工程菌。
Plasmid stability, Antifungal activity, plant\|colonizing ability, UV resistance and inseticidal activity in field were analysed for the engineered \%Pseudomonas fluorescens\%(Pf) strain IPP202. The results indicated that the recombinant plasmid in IPP202 was very stable after successive diluting culturing and after continuous culturing, There was no significant change in the properties beneficial to plants, such as antifungal activity and plant\|colonizing ability as compared with the original strain P303. IPP202 was much more resistant to UV than Bt strain HD73. The control effect in field against cotton boll worm in field was close to that of a locally used Bt\|chemical mixture in normal applied concentration. All the data indicated that the engineered Pf strain was a one with prosperous future after further study.
出处
《微生物学报》
CAS
CSCD
北大核心
2001年第1期3-8,共6页
Acta Microbiologica Sinica
基金
国家"863"高科技项目资助课题(101-03-01-01)
关键词
荧光假单胞菌
遗传工程
生物学特性
Pseudomonas fluorescens
Genetic engineering
Biological properties
