摘要
染色体上引物原位延伸标记在研究染色体结构和基因定位等方面具有重要意义。分别应用随机引物和SOX基因兼并引物在人类染色体上进行了原位延伸标记。结果表明，随机引物延伸在染色体上呈现明暗相间的带纹样特征。SOX基因兼并引物延伸发现了更多的SOX基因位座，并进一步证实该家族基因在基因组中是散在存在的。
Primed in situ labelling (PRINS) contributes to the elucidation of the organization of eukaryotic chromosomes and provides an alternative way to localize gene on chromosomes. Here we present the results of random primer and SOX degenerate primer PRINS on human metaphase chromosomes. The particular band pattern of dark and light was observed by using random primer PRINS. SOX degenerate primer PRINS revealed more SOX gene loci and proved further that SOX genes were not clustered on human chromosomes.
基金
国家自然科学基金!(编号:39770392)&&
