摘要
目的 比较多孔高密度聚乙烯（Medpor）与硅橡胶经紫外线照射前后血清总补体（CH50）活性的变化,探讨紫外线照射对2种材料生物相容性的影响.方法 将Medpor和硅橡胶分别切成大小为1 cm×1 cm×2 mm的块状,部分材料经紫外线照射.选日本大耳白兔24只随机分成4组,每组6只,无菌条件下每只兔距右耳根3～5 cm处做1.5 cm长的弧形切口,分离并去除约1 cm×1 cm软骨,实验1组植入未经紫外线照射的硅橡胶,实验2组植入经紫外线照射的硅橡胶,实验3组为植入未经紫外线照射的Medpor,实验4组植入经紫外线照射的Medpor.于术前3 d和术后1、3、7、14、21、30、60、90 d分别检测CH50含量.结果 4组术区均有不同程度的炎性反应或充血.实验1组和实验2组术后各时间点血清CH50值均低于术前3 d,实验3组和实验4组术后1、3 d低于术前3 d,实验1组和实验2组术后各时间点血清CH50值均分别低于实验3组和实验4组,实验4组术后1、3、7 d血清CH50值高于实验3组,差异有统计学意义（P〈0.05）.结论 紫外线照射可以改善Medpor的CH50值,促进其与组织的相容性,对硅橡胶无明显改善作用.
Objective To compare the change of the total hemolytic complement （CH50） of multihole highDensity polyethylene （Medpor） and silicon elastomers before and after ultraviolet irradiation, and to explore the effect of ultraviolet irradiation on the biocompatibility of silicon elastomers and Medpor. Methods Medpor and silicone elastomers were cut into pieces of 1 cm X 1 ern X 2 mm, and part of the materials were irradiated by ultraviolet. Twenty-four big-ear white Japanese rabbits were randomly divided into 4 groups （n = 6, each）. Each rabbit undertook a 1. 5 ern arc incision, 3 - 5 cm from the right sulcus auriculae posterior under aseptic condition, and then a 1 cm X 1 em auricular cartilage was dissected and removed. Group A was implanted with a silicon elastomer; group B was implanted with a silicon elastomer with ultraviolet irradiation; group C was implanted the Medpor; group D was implanted the Medpor with ultraviolet irradiation. Contents＇ of the CH50 were measured respectively 3 d before the operation and 1 d, 3 d, 7 d, 14 d, 21 d, 30 d, 60 d and 90 d after the operation. Results There were different degrees of inflammatory reaction or hyperemia in surgical areas in the four groups. The values of CH50 each time point after the operation in group A and B were lower than those 3 d before the operation; the CH50 values of 1 d and 3 d after the operation in group C and D were lower than those 3 d before the operation; the CH50 values each time point after the operation in group A and B were respectively lower than those of group C and D; the CH50 values 1 d, 3 d and 7 d after the operation in group D were higher than those in group C, andthe differences were statistically significant （P 〈 0.05）. Conclusion The ultraviolet irradiation can improve the CH50 value of Medpor, augment the biocompatibility of Medpor, but it can not significantly affect the biocompatibility of silicon elastomer.
出处
《解放军医药杂志》
CAS
2014年第1期29-31,共3页
Medical & Pharmaceutical Journal of Chinese People’s Liberation Army
关键词
聚乙烯
弹性聚硅酮类
紫外线
补体溶血活性测定
兔
Polyethylene
Silicone elastomers
Ultraviolet ray
Complement hemolytic activity assay
Rabbit
