摘要
建立了液相色谱法测定维生素C中糠酸方法。采用液相色谱检测，紫外检测器，C18色谱柱，柱温30℃，流动相采用乙腈／磷酸盐缓冲溶液，对检测方法的检出限、定量限、线性、精密度、稳定性进行考察。结果表明该方法线性方程为：Y=51403X＋2142．9，在0．5—10μg·mL^-1内，线性关系良好（r=0．9999）。该方法适合于维生素c中糠酸的测定。
The method of solvent residue determination of vitamin C in liquid chromatography was set up. The method was with HPLC, UV detector, Cls column, the column temperature of 30 ℃, and mobile phase with acetonitrile/ phosphate buffer solution. The detection limit, limit of quantitation, linearity, precision, and the stability of the detection method were investigated. The method of linear equation was Y = 51403X ＋ 2142. 9, 0. 5 - 10 μg . mL^-1, and good linear relationship was r = 0. 9999. This method was suitable for the determination of vitamin C in the bran acid.
出处
《广州化工》
CAS
2014年第2期97-99,共3页
GuangZhou Chemical Industry
关键词
维生素C
糠酸
液相色谱法
Vitamin C
furoic acid
liquid chromatography
