摘要
核桃粕是核桃榨油后的产物,其蛋白质含量丰富,营养价值高。以经过低温压榨后的核桃粕为原料,首先对核桃粕的基本组成进行测定,然后重点对核桃粕酶解的酶种类进行筛选以及对制备核桃多肽的最佳工艺参数进行研究。结果表明,复合酶解核桃粕的最佳酶解比例为中性蛋白酶与植物蛋白酶按2∶1复配,其最佳酶解条件为加酶量8 000 U/g,pH值7.5,温度50℃,酶解时间3 h。在该试验条件下,多肽得率为85.87%,总抗氧化能力为91.73 U/mL。
Walnut meal is the result of oiled walnut, its protein is rich and its nutritional value is high. Therefore, by using the cold pressed walnut meal as raw material, the walnut meal＆#39;s basic composition are measured, walnut meal on the type of enzyme hydrolysis are screened, and on the preparation of walnut peptide optimal parameters are studied. The experiments show that the ratio of neutral protease, plant protease is 2∶1, the hydrolysis ability of composite enzyme is best. Beside, The optimum hydrolysis conditions： enzyme dosage of 8 000 U/g, pH of 7.5 and a temperature of 50 ℃, reaction time is 3 h. In the experimental conditions, the peptide yield 85.87%, total antioxidant capacity 91.73 U/mL.
出处
《农产品加工（下）》
2014年第1期31-34,38,共5页
Farm Products Processing
关键词
核桃粕
复合酶
多肽得率
walnut protein meal
complex enzyme
peptides yield
