摘要
昆虫的线粒体DNA控制区(也称作A+T富集区)已用于昆虫种群的分子系统学研究。测定5个中系家蚕品种、1个野桑蚕地理品系和1个亚洲玉米螟品系的线粒体DNA A+T富集区及nad6基因序列,分析序列结构特征发现家蚕、野桑蚕的A+T富集区含有ATAGA+polyT、微卫星元件(AT)n、polyA以及3个长约32 bp的串联重复序列等4种保守结构,与已报道的其它鳞翅目昆虫类似。使用"A+T富集区+nad6"数据集重建11个家蚕品种(品系)和7个野桑蚕地理品系的系统发育树,结果显示家蚕与中国野桑蚕具有更近的亲缘关系。另外,仅基于A+T富集区序列得到的蚕蛾总科最大似然树与传统分类系统较为一致,提示在一定条件下A+T富集区可用于较高级分类阶元的分子系统学研究。
Insect mitochondrial DNA control region （also known as the A＋T-rich region） has been widely used in molecular systematic studies of insect populations. In this study, the A＋T-rich region of mitochondrial DNAs and nad6 gene sequence from five silkworm （Bombyx mori） varieties of Chinese strain, one geographic strain of B. mandarina, and one strain of Ostrinia fumacalis were sequenced. Sequence structural feature analysis showed that B. mori and B. mandarina had at least four conserved domains, viz. ATAGA＋poly（T） motif, microsatellite element （AT） n, poly（A）, and 3 tandem repeat units （about 32 bp in length） in the A＋T-rich region, being similar with other reported lepidopteran insects. Besides, phylogenetic trees of 11 B. mori varieties/strains and seven geographic strains of B. mandarina were reconstructed using combined dataset of the A＋T-rich region and nad6 gene. The results showed that there is a close re- lationship between B. mori and Chinese B. mandarina. In addition, the maximum likelihood tree of Bombycoidea based on only the A＋T-rich region sequences is consistent with traditional taxonomy, suggesting that A＋T-rich region could be used in higher-level molecular taxonomic studies under certain conditions.
出处
《蚕业科学》
CAS
CSCD
北大核心
2014年第1期18-25,共8页
ACTA SERICOLOGICA SINICA
基金
浙江省农业科学院科技创新能力提升工程项目(No.2012-R06Y01E03)
现代农业产业技术体系专项(No.CARS-22)
关键词
家蚕
线粒体DNA
A+T富集区
序列结构
系统发育
分子标记
Bombyx mori
Mitochondrial DNA
A＋T-rich region
Sequence structure
Phylogenetic analysis
Mol-ecular marker
