摘要
目的 :评价辐射诱发的染色体畸变作为癌患风险评估指标的可行性。方法 :应用双色荧光原位杂交 (FISH)技术检测 10例放疗后及 6例放疗前鼻咽癌患者外周血淋巴细胞染色体畸变并与常规法比较。结果 :放疗后 1～ 10年 ,鼻咽癌患者淋巴细胞染色体易位率、双着丝粒体率仍显著高于放疗前对照组的相应数值 (P <0 .0 0 1)。患者的易位率约为双着丝粒体率的 3 .5倍 ,照后 3年以上患者染色体易位率与照后小于 3年患者的易位率之间差别无显著性 (P >0 .1) ,而照后 3年以上患者染色体双着丝粒体率显著低于照后小于 3年患者的双着丝粒体率 (P <0 .0 0 1)。 结论 :稳定性染色体畸变 (易位 )在照后相当长时间 (10年 )仍可保持较高百分率 ,有望成为癌患风险评估的生物标志。
Purpose:To evaluate the probability of using chromosome aberrations induced by irradiation as a indicator of future cancer risk. Methods:Chromosome aberrations in peripheral blood lymphocytes of 10 irradiated and 6 nonirradiated nasopharyngeal carcinoma patients were studied by two-colour fluorescence in situ hybridization(FISH) and coventional analysis. Results:Both the mean translocation and dicentric frequencies in irradiated patients 1 to 10 years after exposure were still significantly higher than those in nonirradiated patients(P<0.001). For irradiated patients,the translocation frequencies were about 3.5-fold as high as dicentric frequencies. No significant difference was found between translocation frequencies in patients after exposure more than 3 years and that in patients after exposure less than 3 years(P>0.1),but the dicentric frequencies in patients after exposure more than 3 years were significantly lower than that in patients after exposure less than 3 years(P<0.001)Conclusion:The percentages of stable chromosome aberrations(translocations)are still higher even a long time (10 years)after exposure and is expected to be a re1evant biomarker of future cancer risk.
出处
《癌变·畸变·突变》
CAS
CSCD
2001年第1期13-16,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国防科工委基金资助!(Y5 5 732 6 2 )
