摘要
目的研究K-ras基因突变表达与结直肠癌的相关性。方法采用实时荧光定量PCR方法检测89例患者结直肠癌组织中K-ras基因2号外显子12与13编码子突变情况,并结合病理资料进行分析。结果 89例患者结直肠癌组织中K-ras基因突变者为37例,突变率为41.6%。其中12编码子突变为29例,突变率为32.6%。13编码子突变为8例,突变率为9.0%。K-ras基因突变与肿瘤位置、分化程度无明显相关性(P>0.05),与浸润深度、淋巴结转移、肝转移有相关性(P<0.05)。淋巴结转移越多K-ras基因突变率越高,有肝转移者K-ras基因突变率高。结论 K-ras基因突变在结直肠癌的发生、发展中起重要作用,与浸润深度、淋巴结转移和肝转移密切相关。
Objective To investigate the correlation between K-ras gene mutation and colorectal cancer. Methods The specimens of 89 patients with colorectal cancer were collected. K-ras mutations of exon 2 were examined by real-time fluorescence quantitative PCR at codon 12 and codon 13. The results were correspondingly analysed with clinical pathological data. Results K-ras mutations were found in 37 cases. The mutation rate was 41.6%. In 29 cases,mutations occurred in codon 12, and the mutation rate was 32.6%. Mutations occurred in codon 13 in 8 specimens with 9.0% mutation rate. No correlation between K-ras mutation and tumor location or differentiation extent was observed. The K-ras mutation rate was related to the infiltrating degree, lymph node metastasis and hepatic metastasis. Conclusion K-ras mutation plays an important role in the carcinogenesis and development of colorectal cancer,which is closely associated with invaded lymph nodes and hepatic metastasis.
出处
《实用肿瘤杂志》
CAS
2014年第1期33-35,共3页
Journal of Practical Oncology
关键词
结肠肿瘤
直肠肿瘤
K-RAS基因
突变
肿瘤转移
colonic neoplasms
rectal neoplasms
K-ras genes
mutation
neoplasm metastasis
