摘要
目的探讨紫花地丁用于炎症性疾病的疗效机制。方法采用紫花地丁水煎剂灌胃干预清洁级SD大鼠常规制备含药血清,按一定浓度于96孔板中与小鼠腹腔巨噬细胞共同培养,24 h后以Griess比色法检测培养上清中NO含量,ELISA法检测培养上清中TNF-α和IL-6含量。结果与相同浓度的正常血清对照组比较,在5%～20%的紫花地丁含药血清作用下:1)未经LPS活化的巨噬细胞TNF-α、NO分泌增加(P<0.05或P<0.01),而IL-6分泌则降低(5%含药血清组)(P<0.01)或增加(10%含药血清组)(P<0.01);2)经LPS活化的巨噬细胞TNF-α、NO分泌降低(P<0.05或P<0.01),而IL-6分泌则降低(10%含药血清组)(P<0.01)或增加(20%含药血清组)(P<0.01);与同浓度组未经LPS活化的巨噬细胞比较:1)正常血清5%～20%各浓度组经LPS活化的巨噬细胞TNF-α、IL-6及NO分泌增加(P<0.05或P<0.01);2)紫花地丁含药血清5%～20%各浓度组经LPS活化的巨噬细胞NO分泌量显著降低(P<0.01),TNF-α分泌量在5%含药血清浓度组降低(P<0.01),而在10%和20%浓度组升高(P<0.01),IL-6分泌量在5%含药血清浓度组降低(P<0.01),而在20%浓度组升高(P<0.01)。结论紫花地丁含药血清对巨噬细胞产生TNF-α、IL-6和NO的干预作用,可能是其调控炎症反应过程的重要机制。
In order to investigate the efficacy of viola treatment on inflammatory diseases, we prepared the serum containing viola and blank serum, which were then cocuhured at a certain concentration with mouse peritoneal macrophages in 96-well plates. Twenty four hours later, NO content was determined in the culture supernatant by Griess colorimetry, while TNF--α and IL-6 content were determined in the culture supernatant by ELISA. Compared with the same concentration of normal serum group, 5%-20% serum containing viola could promote unactivated macrophages to secret TNF-αand NO （P〈 0.05 or P〈 0.01）, while elevate （5% viola serum group, P〈0.01） or inhibit （10% viola serum group, P〈 0.01） IL-6 secretion of unactivated macrophages; as for LPS-activated macrophages, 5%- 20% serum containing viola could down-regulate the secretion of TNF--α and NO（P〈0.05 or P〈0.01）, while decrease secretion of IL-6（10% containing serum group, P〈0.01） or increase secretion of IL--6 （20% containing serum group, P〈 0.01）. Compared with the same concentration of unactivated macrophages group, LPS-activated macrophages in 5%- 20% normal serum group secreted more TNF-α, IL-6, and NO （P〈0.05 or P〈O.01）, while LPS-activated macrophages secreted less NO （P〈0.01） in 5%-20% serum containing viola, less TNF-α in 5% serum containing viola （P〈0.01）, and more TNF-α in 10% and 20% serum containing viola group （P〈 0.01）, less IL-6 in 5% serum containing viola group （P〈0.01）, and more IL-6 in 20% serum containing viola group （P〈 0.01）. All the results come to the conclusion thatserum containing viola could intervene TNF-α, IL-6 and NO production of macrophages, which may be the mechanism of viola regulating inflammatory response.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2014年第1期53-56,共4页
Immunological Journal
基金
贵州省委组织部人才办特助项目(TZJF-2009年39号)
贵州省科技厅国际科技合作项目(黔科合外G字[2010]7014号)
贵阳中医学院研究生创新计划项目(ZYYCX12015)
