摘要
以多年生黑麦草品种玲珑的愈伤组织为受体材料,建立了适用于农杆菌介导的转基因组织培养体系,并对耐盐相关基因SOS1、SOS2、SOS3、CBL10和BAR基因进行转化。结果表明,以成熟种子作为外植体诱导胚性愈伤的最佳培养基为MS+7mg/L 2,4-D+500mg/L水解酪蛋白,最佳分化培养基为2.0mg/L 6-BA+0.2mg/L 2,4-D;愈伤筛选和再生苗筛选的最佳除草剂浓度为5mg/L PPT;筛选出的最佳侵染时间为20min,共培养时间为2d,建立了多年生黑麦草有效的遗传转化体系。
Embryonic callus of Perennial Ryegrass ＇Linglong＇ were used as acceptor material, an effi- cient Agrobacterium-mediated transformation tissue culture system was established, and SOS1, SOS2, SOS3, CBLIO and BAR genes which are related to salt resistance were transformed. The results showed that the mature seeds were used as explants to induce embryonic callus, the best medium was MS q- 7mg/L 2, 4-D＋ 500mg/L CH, and the best differentiation medium was 2.0mg/L 6-BAq- 0.2mg/L 2,4-D; the best herbicide concentration was 5mg/L for callus screening and regeneration seedling screening; The best infection time was 20 minutes, co-culture time was 2 days, an efficient Agrobaeterium-mediated transfor- mation system was established.
出处
《中国草地学报》
CSCD
北大核心
2014年第1期13-17,共5页
Chinese Journal of Grassland
基金
国家"973"项目子课题"鉴定由调控网络重要节点构成的调控单元
并进行耐盐碱作物分子育种设计"(2012CB114204)
"十一五"国家科技支撑计划资助项目"荒漠草原区农牧复合生态系统构建与可持续利用技术集成试验与示范"(2011BAC07B03)
宁夏农发办项目[NTKJ-2013-09(1)]
关键词
多年生黑麦草
遗传转化体系
农杆菌
Perennial ryegrass
Genetic transformation system
Agrobacterium
