摘要
以‘洛阳包头’大白菜无菌苗叶柄为外植体,研究了大白菜通过直接器官发生建立植株再生体系的方法。结果表明:不同激素组合对‘洛阳包头’大白菜无菌苗顶芽增殖、大白菜叶柄不定芽的诱导以及再生植株生根的影响不同;配方MS+BA 1.0mg/L+IBA 0.3mg/L的培养无菌苗顶芽基增殖率最高,达86.7%;配方MS+BA 2.5mg/L+IBA 0.3mg/L对大白菜叶柄不定芽的诱导效果较好,诱导率为76.7%;配方MS+IBA 1.0mg/L诱导再生植株生根率最高,达90.0%。
Taking the no vaccine petiole of Brassica pehinensis C／T. ＇Luoyang baotou＇ as explant, the cabbage plant regeneration system was established by direct organogenesis. The results showed that the effects of different plant hormones combinations at different concentrations on seedlings propagation, adventitious buds induction from petiole explants and seedling rooting were different. The optimal medium for propagation of seedlings was MS supplemented with BA 1. 0 mg/L -HBA o. 3 mg/L,and the induction rate was 86. 7 %; the highest adventitious buds induction frequency was 76.7% by using MS supplemented with BA 2.5 mg/L＋ IBA 0.3 mg/L, the optimal medium for rooting was MS supplemented with IBA 1. 0 mg/L and the rooting rate was 90.0%.
出处
《北方园艺》
CAS
北大核心
2014年第4期86-90,共5页
Northern Horticulture
基金
国家自然科学基金资助项目(31100511)
河南省高校青年骨干教师计划资助项目(2010GGJS-161)
关键词
'洛阳包头’大白菜
叶柄
组织培养
植株再生
Brassica pehinensis cv. ＇Luoyang baotou＇
petiole
tissue culture
plantlet regeneration
