摘要
以榕树(Ficus microcorpa)为试材,研究了冬季低温条件下不同浓度赤霉素(GA3)(0、15、150mg/L)和硫酸铵((NH4)2SO4)(0、20、200g/L)处理15、30、45d对活性氧代谢酶系统的愈创木酚过氧化物酶(G-POD)活性、抗坏血酸过氧化物酶(ASA-POD)活性、过氧化氢酶(CAT)活性、超氧化物歧化酶(SOD)活性和非酶系统丙二醛(MDA)含量的影响。结果表明:经GA3和(NH4)2SO4处理后可保持相对较高的抗氧化酶(POD、CAT、SOD)活性,削弱MDA含量的积累。在低温胁迫下随着GA3浓度的增加,POD活性、CAT活性和SOD活性呈先升高后降低的趋势;随(NH4)2SO4浓度的增加,POD活性、CAT活性和SOD活性呈增高的趋势,而MDA含量下降。含200g/L(NH4)2SO4的各处理15d后G-POD活性均表现得极为明显,15mg/L GA3+200g/L(NH4)2SO4处理下,AsA-POD活性均达到最高,15mg/L GA3+200g/L(NH4)2SO4处理30d后SOD活性持续上升并达峰值,15mg/L GA3+20g/L(NH4)2SO4处理15d后达到差异极显著水平(P<0.01);受GA3和(NH4)2SO4处理后榕树叶片MDA含量逐渐下降,在处理15d时达到最低;且30d和45d处理后整体MDA含量较15d有所升高。
Taking Ficus microcorpa as test material ,different concentrations of GA3 （0,15,150 mg/L） and （NH4）2 SO4 （0, 20,200 g/L） under low temperature in winter after treatment 15,30,45 d on active oxygen metabolism enzymes guaiacol peroxidase （G- POD） activity, ascorbate peroxidase （ASA- POD） activity, catalase （CAT） activity, superoxide dismutase enzyme （SOD） activity and （MDA） content were studied. The results showed the application of gibberellic acid and ammonium sulfate increased the root activity, reduced MDA content in leaves during recovery time after chilling. Under low temperature stress increased with the GA3 ,POD activity, CAT activity and SOD activity were increased firstly and then decreased. With the increase of ammonium sulfate,POD activity,CAT activity and SOD activity were increased-and MDA content was decreased. 200 g/L （NH1 ）2SO4 for 15 d.and G-POD activity was very significant performance. In the treatments of 200 g/L （NH4 ）2SO4 and 15 mg/L GA3 ,ASA-POD activity was the highest. 15 mg/L GA3 and 200 g/L （NH4 ）2SO4 treatment at 30 d, SOD activity continued to increase and reached peak. 15 mg/L GA3 and 20 g/L （NH4）2 SO4 for 15 d.it was to reach a significant level（P〈0.01）. After the treatment of GA3 and （NH4）2 SO4,.content of MDA content was decreased. It was to a minimum after the treatment of 15 d. And after the treatment of 30 d and 45 d, the overall MDA content was increased which compared with 15 d.
出处
《北方园艺》
CAS
北大核心
2014年第4期53-57,共5页
Northern Horticulture
关键词
赤霉素
硫酸铵
榕树
活性氧代谢
酶
丙二醛
gibberellic acid （GA3 ）
ammonium sulfate （ NH4 ）2 SO4 ）
Ficus microcorpa
metabolism of activity oxygen
enzyme
MDA
