摘要
[目的]考察制备过程中工艺优化对人凝血因子Ⅷ制备的影响.[方法]以人冰冻血浆经过融解,0～4℃离心,收集的冷沉淀为原始材料,通过调整不同倍数的溶解液、选择不同的抗凝剂、调节不同的酸沉pH,观察蛋白液的稳定性,检测蛋白液中蛋白质含量及凝血因子Ⅷ的活性.结果：加入冷沉淀重量5倍的溶解液,用4 IU/ml肝素钠抗凝,调节酸沉的pH在6.2 ～6.4范围内,蛋白液的稳定性最佳,人凝血因子Ⅷ的活性最高.[结论]通过优化人凝血因子Ⅷ提取过程的参数,可以显著提高人凝血因子Ⅷ的稳定性,降低杂蛋白浓度,保护Ⅷ因子的活性.
[Objective] To investigate the effects of optimization process parameter to preparation Human Coagulation Factor Ⅷ.[Method] After thawing frozen plasma,centrifuging at 0-4℃ and collecting the cryoprecipitate as a raw material,by adjusting the different multiples of the dissolving liquid,selecting different anticoagulants,and regulating the different acids pH value to observe the protein solution stability,and detect protein content and human coagulation factor Ⅷ activity.[Result] Adding cryoprecipitate 5 times by weight of the dissolving liquid,4IU/ml sodium heparin anticoagulant,adjusting the acid precipitation pH 6.2-6.4,the stability of the protein solution is the best,and human coagulation factor Ⅷ has the highest activity.[Conclusion] By optimizing the parameters of human coagulation factor Ⅷ extraction process can significantly improve the stability of human coagulation factor Ⅷ and reduce the hybrid protein concentration,protect Ⅷ factor activity.
出处
《安徽农业科学》
CAS
2013年第30期12043-12044,12048,共3页
Journal of Anhui Agricultural Sciences
关键词
冷沉淀
人凝血因子Ⅷ
酸沉
Cryoprecipitate
Human coagulation factor Ⅷ
Acid precipitation
