摘要
目的:探讨西妥昔单抗(C225)与奈达铂(NDP)联合应用对Hela细胞增殖的影响。方法:培养宫颈癌Hela细胞,分为C225单药组(C225的质量浓度依次是10、20、40、60、80和160 mg/L)、NDP单药组(NDP的质量浓度依次是1、5、10、20、40和60 mg/L)、C225+NDP联合用药组(40 mg/L C225分别联合不同质量浓度的NDP)。每个质量浓度分别作用Hela细胞24、48和72 h后用MTT法检测细胞增殖抑制率。结果:C225单药不能抑制Hela细胞增殖,随着药物质量浓度的增加和作用时间的延长,细胞增殖抑制率的变化差异无统计学意义(P>0.05)。NDP单药对Hela细胞有明显抑制作用,随着药物质量浓度的增加(F=3 200.480,P<0.001)和作用时间的延长(F=3 057.250,P<0.001),细胞的增殖抑制率逐渐增加,浓度和时间存在交互作用(F=99.701,P<0.001)。C225联合NDP用药的细胞增殖抑制率明显高于对应的C225或NDP单药,差异有统计学意义(F=39.507,P<0.001)。结论:C225可增强Hela细胞对NDP化疗的敏感性。
Aim ： To study the effects of nedaplatin（NDP） combined with cetuximab（ C225 ） on the proliferation of cervical carcinoma Hela cell line. Methods： The Hela cells were allocated into C225 groups, NDP groups and combination groups of C225 and NDP（C225 ＋ NDP groups）. The concentration of C225 groups was 10,20,40,60,80 and 160 mg/L, the concentration of NDP groups was 1,5,10,20,40 and 60 rag/L; and that of C225 ＋ NDP groups was 40 mg/L C225 plus NDP at different concentrations. Hela cells were treated for 24 h, 48 h, 72 h. The inhibitory effects of C225 and NDP on the Hela cells were evaluated by MTT. Results： C225 had no inhibitive effects on Hela cells with the increased concentra- tions and time （ P 〉 0.05 ）. NDP could significantly inhibited the growth of Hela ceils, and the inhibition showed concentra- tion and time dependence and interaction（ F =3 200. 480,3 057. 250, and 99. 201 ,P 〈0. 001 ）. The combination of C225 and DNP had better inhibitive effects on Hela ceils than single use of C225 or NDP（ F = 39. 507 ,P 〈 0. 001 ）. Gonelusion ： C225 could enhance the inhibition effects of NDP on Hela cell line.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2014年第1期99-102,共4页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省卫生厅科技攻关项目201203043
