摘要
目的研究肿瘤型丙酮酸激酶(tM2-PK)在结直肠癌(CRC)患者中的表达及相关性,为CRC的早期诊断、预后判断和治疗后随访,寻求一种简便可靠、无创的检测方法。方法分别应用免疫组化(IHC)法和酶联免疫吸附(ELISA)法检测68例CRC患者癌组织和术前血清tM2-PK水平,并与40例结直肠息肉患者和40例健康体检者作比较。结果①CRC组血清tM2-PK水平明显高于结直肠息肉组、正常组(P<0.05),其表达与肿瘤大小、Duke分期均显著相关;②CRC组织中tM2-PK阳性表达率明显高于结直肠息肉组织和癌旁正常组织(P<0.05),其表达与肿瘤大小、分化程度、Duke分期均显著相关;③血清与癌组织的tM2-PK水平密切相关(r=0.357,P=0.003)。结论血清和癌组织tM2-PK测定对CRC诊断、预后评估及监测有一定的临床意义。
Objective To investigate the expression and clinical signification of tumor M2 pyruvate kinase in pa-tients with CRC, and to try to find a reliable and atraumatic method to predict the progress and pronosis in the CRC. Methods ELISA method was used to determine the serum levels of tM2-PK in 68 patients with CRC,40 pa-tients with colorectal polyps and 40 cases of normal subjects; immunohistochemistry （ IHC ） methods were respec-tively used to detect the expression of tM2-PK in tissues. Results ① The level of serum tM2-PK in patients with CRC was significantly higher than the group of patients with polyps and the normal subjects （ P＆lt;0.05 ） . The serum tM2-PK levels was closely correlated to tumor size and Duke stage.②The positive rate of tissue tM2-PK was signif-icantly higher in CRC than in colorectal polyps and normal subjects （ P＆lt;0.05 ） . Tissue tM2-PK levels was closely correlated to tumor size,Duke stage and differentiation grade. ③Serum tM2-PK levels was correlated to tissue tM2-PK levels （r=0.357,P=0.003）. Conclusion Combined detection of expression of tM2-PK can provide the basis theory for the diagnosis and prognosis evaluation of CRC.
出处
《安徽医科大学学报》
CAS
北大核心
2014年第2期236-239,共4页
Acta Universitatis Medicinalis Anhui
基金
国际科技合作计划(编号:1303063016)
