摘要
目的:研究青海世居汉族人群乳腺癌易感性与X射线损伤修复交叉互补基因1(XRCC1)C26304T位点基因多态性的相关关系。方法:应用聚合酶链反应-限制性片段多态性(PCR-RFLPs)方法检测青海世居汉族人群30例乳腺癌患者(乳腺癌组)及30例乳腺良性病变患者(对照组)XRCC1C26304T基因多态性分布情况。同时运用统计学方法对XRCC1 C26304T的基因型和基因频率进行分析。结果:XRCC1 C26304T基因CC、CT、TT基因型在对照组中分别是56.7%、33.3%、10%,而在乳腺癌组中分别是50.0%、36.7%、13.3%。TT基因型在对照组及乳腺癌组中,相对于CT、CC基因组表达较低,但是对照组和乳腺癌组间并没有明显差异(P>0.05)。经过分析,C、T基因频率在对照组中是0.733、0.267,而在乳腺癌组中是0.683、0.317,两组比较,C、T基因频率也无明显差异(P>0.05)。结论:XRCC1 C26304T基因多态性可能与青海世居汉族人群乳腺癌易感性无关。
Objective： To investigate the relation between polymorphisms of XRCC1 and Han patients with breast cancer in Qinghai area. Methods：The polymerase chain reaction - restriction fragments length polymor- phisms （PCR- RFLPs） technique was applied to analyze the XRCC1 C26304T in breast cancer group （n = 30） and the control group （ Patients with benign breast lesions, n = 30）. The genotype and the gene frequency of the XRCC1 C26304T were analyzed statically. Results：The frequency of XRCC1 C26304T CC, CT, TT genotype is 56.7%,33.3%,10.0% respectively in the control group; in the breast cancer group, they is 50.0% ,36.7% andl3.3%, respectively. The TT genotype expression is lower compared with the CT and CC genome in the control group and breast cancer group, but there were no significant difference in both groups （P 〉 0.05 ）. The frequency of C and T gene in the control group was 0.733 and 0.267, and was 0.683 and 0.317 respectively in breast cancer group ; there were no significant difference in the C and T gene frequency between the two groups （P 〉 0.05 ）. Conclusions： XRCC1 C26304T polymorphisms may not be correlated with the susceptibility of Han patients with breast cancer in Qinghai area.
出处
《高原医学杂志》
CAS
2013年第2期4-7,共4页
Journal of High Altitude Medicine
