摘要
目的:建立核酸特异、快速、敏感的Taqman探针实时定量PCR检测方法筛选疑似人博卡病毒(HBoV)感染者鼻咽拭子临床样本中的阳性样本。方法:比对编码HBoV-sh9的基因序列,选取保守区序列设计引物和探针,建立Taqman PCR检测人博卡病毒的阳性率的方法,进行特异性实验,检测214例临床样本。结果:所建立的Taqman PCR方法可以用于检测人博卡病毒的阳性率,对所收集得到的214例急性上呼吸道感染者的咽拭子标本进行检测,结果显示,荧光定量PCR检测出8例阳性,其阳性率为3.74%。结论:建立了HBoV TaqMan探针实时定量PCR检测方法,可以快速灵敏的检测出阳性人博卡病毒,为临床治疗提供快速可靠的诊断依据。
Objective. To establish a specific, rapid, sensitive TaqMan probe based real-time quan- titation PCR （Q-PCR） for detecting positive rate of nasopharyngeal swab of suspected person with HBoV. Methods： Comparing the gene sequences coding HBoV9, the primers and probe was designed according to the conservative sequences. TaqMan-PCR were established to detect the HBoV of 214 clinical samples. Results： The established method of Taqman PCR can be used for HBoV positive rate detection. Among 214 nasopharyngeal swab specimens screened for the presence of HBoV by Taqman-PCR, 8 specimens were i- dentified positive for HBoV. The positive detection rate of HBoV by Q-PCR was 3.74 %. Conclusions：A real-time Q-PCR assay for detection and quantitation of HBoV has been developed. The assay can detect HBoV rapidly and sensitively. This assay can be applied for surveillance and clinical diagnosis of HBoV.
出处
《海南医学院学报》
CAS
2014年第3期289-292,共4页
Journal of Hainan Medical University
基金
国家青年科学基金项目(81101306)~~
