摘要
目的建立高效液相色谱与原子荧光光谱联用测定水产品中甲基汞及其他形态汞的检测方法。方法样品经盐酸超声提取后离心过滤，以5％乙腈4．62g／L乙酸铵-1．2g／L半胱氨酸溶液作流动相，经反相C18色谱柱分离后无需氧化和紫外消解，直接由原子荧光光谱仪检测，外标法定量。结果甲基汞、乙基汞和无机汞的最佳线性范围为0～50μg／L，r〉0．9990，检出限为0．5～1．5μg／L，精密度RSD〈6％，加标回收率为78％～120％，鱼肉标准物质中甲基汞测定值在参考值范围内。结论该法操作简便灵敏，结果准确可靠，适合于水产品中汞形态的测定。
Objective To establish a method for measuring methylmercury and other mercury species in aquatic products by HPLC-AFS. Methods The samples were extracted and centrifuged after hydrochloric acid uhrasoundtreatment. 5% of Acetonitrile, 4. 62 g/L ammonium acetate and 1.2 g/L cysteine solution was used as mobile phase. Then the samples, being tested with AFS after C18 seperated, were quantitatively determined by external standard method. There was no need of oxidization or hraviolet photolysis. Results The best linear range of methylmercury, ethylmercury and inorganic mercury was 0 - 50 μg/L with correlation coefficients above 0. 999 0, the detection limit was 0.5 - h 5 μg/L, the precision relative standard deviation was less than 6% , and recovery of spiked samples was 78% - 120%. Methylmercury measurement of standard substance in fish was within the reference range. Conclusion This method was simple, sensitive, accurate and suitable for the determination of mercury species in aquatic products.
出处
《中国食品卫生杂志》
北大核心
2014年第1期58-62,共5页
Chinese Journal of Food Hygiene
基金
深圳市科技计划项目(200903119)
