摘要
采用循环伏安（CV）、线性扫描伏安（LSV）和示差脉冲伏安（DPV）等方法研究了8-羟基脱氧鸟苷（8-OHdG）在壳聚糖（Chi）/石墨烯（GR）修饰的玻碳电极（GCE）上的电化学行为，8-OHdG在该修饰电极上氧化峰电流与其浓度在3.5×10^-7-1.4×10^-4 mol/L范围内呈良好的线性关系，检测限为6.4×10^-8 mol/L（S/N=3）。 将Chi/GR/GCE用于检测DNA氧化损伤，8-OHdG在修饰电极上的氧化峰电流与损伤的DNA质量浓度在10~300 mg/L范围内呈良好的线性关系，损伤DNA检出限为0.026 mg/L（S/N=3）。
The electrochemical behavior of 8-hydroxydeoxyguanosine（8-OHdG） at the chitosan（Chi）/graphene（GR） modified glassy carbon electrode（GCE） was investigated by cyclic voltammetry, linear sweep voltammetry and differential pulse voltammetry. A linear relationship between the oxidative peak current and concentrations of 8-OHdG at the modified electrode was established in the range of 3.5×10^-7-1.4×10^-4 mol/L with a detection limit of 6.4×10^-8 mol/L（S/N=3）. Chi/GR/GCE was used for the detection of DNA oxidative damage, it showed that the oxidative peak current of 8-OHdG at the modified electrode was linear with concentration of damaged DNA in the range of 10-300 mg/L with a detection limit of 0.026 mg/L（S/N=3）.
出处
《应用化学》
CAS
CSCD
北大核心
2014年第2期200-205,共6页
Chinese Journal of Applied Chemistry
基金
江苏高校优势学科建设工程资助项目(164320H106)~~
关键词
羟基脱氧鸟苷
壳聚糖
石墨烯
DNA氧化损伤
电化学检测
hydroxydeoxyguanosine, chitosan, graphene, DNA oxidative damage, electrochemical detection
