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杉木采样时间及DNA提取方法的研究 被引量:1

Study of sampling time and means of DNA extracting of cunninghamina lanceolata
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摘要 从材料中提取DNA时,必须因材而异,杉木材料中含有较多的酚类等粘性物质。对杉木的采样时期及其 DNA提取方法进行的研究表明:5月份采取杉木新芽 3 g,用 CTAB洗净法可以获得浓度和纯度最高的DNA。 The means of extracting DNA are different from research materials. Study of sampling time and means of extracting DNA of cunninghamia lanceolata have been discussed in this paper. The results show that the highest concentration and purity DNA can be obtained when sampling in May and by the means of CTAB washing.
出处 《湖北林业科技》 2000年第C00期4-7,共4页 Hubei Forestry Science and Technology
关键词 杉木 采样时期 DNA 提取方法 遗传育种 冷冻缓冲液法 CTAB洗净法 CTAB法 Cunninghamia lanceolata DNAI extracting
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  • 2陈小强,王春国,李秀兰,宋文芹,陈瑞阳.植物DNA甲基化及其表观遗传作用[J].细胞生物学杂志,2007,29(4):519-524. 被引量:16
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  • 7Miura K, Agetsumaa M, Kitanoa H, Yoshimurab A, Matsuokaa M, Jacobsenc S E, Ashikaria M. 2009. A metastable DWARF1 epigenetic mutant affecting plant stature in rice. Proc Natl Acad Sci, 106 (27): 11218 - 11223.
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