摘要
目的探讨表皮粘附因子epithelialcadherinEcd表达与涎腺肿瘤的分化和临床生物学行为的关系。方法采用Ecd多克隆抗体的过氧化物酶的免疫组化检测技术。Ecd表达以细胞浆内有棕黄色颗粒为阳性,染色强度则以正常腮腺组织阳性细胞呈现的棕黄色染色特征(++)为标准。结果2例正常涎腺组织Ecd在腺泡细胞尤其在腺管系统和腺泡—腺管单位有明显表达。5例良性混合瘤均为正常表达。涎腺癌47例中,39例Ecd表达减弱或消失。高恶性涎腺癌与低恶性涎腺癌的Ecd表达有明显的差异。术前累及面神经和皮肤的11例均为Ecd表达减弱或消失。Ecd表达的减弱或消失与涎腺癌的复发、转移和5年生存率有明显的关系。结论:涎腺肿瘤组织的Ecd检测有可能作为判别其临床生物学行为和预后的指标。
Objective:The current study was designed to exp lore the relationship between the ep ithelial cadherin(E-cd)expression and clinical appearance s of salivary tumor.Method:The paraffin embedded tissue specim ens were studied immunohistochemically using the ployclone antibody against E-cd and ABC method.The E-cd expressio n level was determined semiquantatively.The s emi-quantitative parameters of E-c d expression were analyzed retrospectively with the clinical information.Result:The E-cd positive expressions were n ormal(++)in two cases of normal salivary tissu e,5cases of benign mixed tumor and parac ancerous salivary tissue,39cases o f of 47malignant salivary malignancies it was decreased even to no E-cd expression.There was a significant correlatio n between decreased E-cd expression and preoperative clinical appearances,postoperative survival,recurren ce or metastasis.Conclusion:The E-cd expression level may be a useful prognostic parameter for salivary malignancies.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2001年第2期191-194,共4页
Chinese Journal of Cancer
关键词
涎腺肿瘤
表皮粘附因子
免疫组织化学
预后
Salivary malignancies
Epithelial cadherin
Immunohistochemistry
P rognosis
