摘要
目的探讨乳腺癌细胞化疗继发耐药后细胞迁移能力的变化,并筛选出相关基因。方法以MCF-7细胞株为亲本细胞。采用阿霉素(ADM)低浓度持续加量诱导法建立对ADM耐药的人乳腺癌细胞株MCF-7/ADM。应用ATP-TCA药物敏感检测法和xCELLligence/RT-CES实时细胞电子分析系统检测人乳腺癌细胞株MCF-7、MCF-7/ADM对多种化疗药物的耐药情况及细胞增殖能力。应用Transwell实验和xCELLligence/RT-CIM实时细胞电子分析系统检测人乳腺癌细胞株MCF-7、MCF-7/ADM的细胞迁移能力。应用比较基因组芯片筛选出人乳腺癌细胞株MCF-7、MCF-7/ADM DNA拷贝数4倍差异的基因。结果撤药培养150d后,MCF-7/ADM细胞较亲本细胞MCF-7的ADM耐药指数为72.9,对其他化疗药物无明显的交叉耐药性。MCF-7/ADM细胞迁移能力较MCF-7明显降低(P<0.05)。MCF-7/ADM有12种基因DNA拷贝数是MCF-7的4倍,MCF-7有6种基因DNA拷贝数是MCF-7/ADM的4倍。结论乳腺癌细胞化疗继发耐药伴随细胞迁移能力明显降低,其相关基因的DNA拷贝数亦有明显差异。
Objective To investigate the change of the cell migration ability of breast cancer cells with acquired resistance to chemotherapy, and screen the related genes. Methods MCF-7 cells were used as the parental cells, and human breast can- cer cell line MCF-7/ADM were established with continuous low concentration of ADM. The chemotherapy resistance of MCF-7 and MCF-7/ADM cell was detected by ATP-TCA drug sensitive test and xCELLligence/RT-CES real-time cerl electronic analysis system. The cell migration of MCF-7 and MCF-7/ADM cell was detected by Transwell test and xCELLligence/RT-CIM real-time celt electronic analysis system. 4 times the difference of DNA copies of Genes in MCF-7 and MCF-7/ADM cell were screeninged by comparative genomic chip. Results The drug resistant index of MCF-7/ADM cell kept on 72.9 times over MCF-7 cell after cultured in the medium （without ADM） for 150 days. MCF-7/ADM had no obvious cross-resistance to other chemotherapy drugs. The cell migration of MCF-7/ADM reduced significantly than that of MCF-7 （P〈0.05）. The DNA copies of 12 genes of MCF-7/ADM cell are 4 times more than that of MCF-7, while the DNA copies of 6 genes of MCF-7 cell are 4 times more than that of MCF-7/ADM Conclusion The migration ability of breast cancer cells was significantly reduced with acquired chemotherapy resistance, and the DNA copies of some genes are also obvious different.
出处
《浙江医学》
CAS
2013年第23期2050-2053,共4页
Zhejiang Medical Journal
基金
宁波市自然科学基金项目(2009A610179)
关键词
乳腺癌
阿霉素
化疗耐药
细胞迁移
Breast cancer ADM Chemotherapy resistance Cell migration
