摘要
根据ＴＮＦα转换酶（ＴＮＦα ｃｏｎｖｅｒｔｉｎｇ ｅｎｚｙｍｅ，ＴＡＣＥ）ｃＤＮＡ序列和 ＴＡＣＥ分子结构特征设计并合成引物，采用逆转录 ＰＣＲ（ＲＴ－ＰＣＲ）技术，首次从健康人脾贴壁细胞中扩增出 ＴＡＣＥ ｃＤＮＡ解整合素结构片段（ｄｉｓｉｎｔｅｇｒｉｎ ｄｏ－ｍａｉｎ），并用大肠杆菌脂多糖（ＬＰＳ）刺激人脾贴壁细胞，观察其对 ＴＡＣＥ ｍＲＮＡ表达的调节作用。结果表明：不同健康人脾贴壁细胞中均存在 ＴＡＣＥ ｍＲＮＡ的自然表达，并且 ＬＰＳ对该表达具有上调作用，其上调程度与 ＬＰＳ刺激时间呈正比关系。
A pair of primers were designed and synthesized according to TNF-alpha converting enzyme (TACE) cDNA sequence and characteristics of molecular structure. By using the technique of reverse transcrip- tion-PCR (RT-PCR), the disintegrin domain of TACE cDNA was firstly amplified from human spleen adhen- sion cells induced by LPS. The effects of stimulation of LPS on the level of TACE mRNA expression sere ob- served. The results showed that TACE mRNA was normally expressed in human spleen. Furthermore, the level of TACE mRNA expression was up-regulated by LPS stimulation in a time-dependent manner.
出处
《同济医科大学学报》
CSCD
2000年第6期564-566,共3页
Acta Universitatis Medicinae Tongji
基金
国家自然科学基金资助项目(No.39630320)
