摘要
在7.5 L发酵罐中研究pH值及流加苯丙酮酸和葡萄糖对副干酪乳杆菌W2分批发酵产苯乳酸(phenyllactic acid,PLA)的影响。结果表明:发酵液初始pH值、底物葡萄糖和苯丙酮酸对菌体生长和产物产量都有影响。控制发酵液pH值为6.5,采用间歇流加苯丙酮酸及连续流加苯丙酮酸和葡萄糖,发酵36 h后PLA产量分别达到1.148 g/L和2.121 g/L,苯丙酮酸的转化率分别为62.19%和47.2%,与分批发酵相比分别提高41.72%和161.53%。
In this paper, the effects of initial medium pH and feeding of the substrates glucose and phenylpyruvic acid （PPA） on the production of phenyllactic acid （PLA） by Lactobacillus paracasei W2 in a 7.5 L fermentor were studied. The results indicated that all three factors generated corresponding effects on cell growth and PLA production. Two substrate feeding strategies, intermittent feeding of PPA and continuous feeding of both glucose and PPA were performed and the initial medium pH was set to 6.5. After 36 h of fermentation under these conditions, PLA production reached 1.148 g/L and 2.121 g/L, respectively, and the conversion rate of PPA reached 62.19% and 47.2%, respectively. In addition, in comparison with fed- batch fermentation, the PLA yield was enhanced by 41.72% and 161.53%, respectively.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2014年第1期163-166,共4页
Food Science
基金
国家农业科研成果转化资金项目(2013GB2C100176)
江苏省科技支撑计划项目(BE2011393)
苏州科技支撑计划项目(SN201130)
常熟市科技发展计划项目(CN201220)
关键词
副干酪乳杆菌
苯乳酸
苯丙酮酸
底物流加
Lactobacillus paracasei
phenyllactic acid
phenylpyruvic acid
substrate feeding
