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MicroRNA-206可能通过调控Cx43表达调节人乳腺癌细胞的转移能力 被引量：6

Inhibitory effects of microRNA-206 on migration and invasion of breast cancer by targeting Cx43
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摘要 目的通过干预人乳腺癌细胞株MCF-7和MDA-MB-231中微小RNA206(microRNA-206,miR-206)的表达,观察连接蛋白43(connexin43,Cx43)的表达以及细胞在增殖、迁移和侵袭能力等方面的变化。方法使用荧光定量RTPCR(qRT-PCR)检测正常乳腺细胞MCF-10A和MCF-7、MDA-MB-231细胞中miR-206的表达水平。通过构建慢病毒载体转染MCF-7及MDA-MB-231细胞,分别升高和降低miR-206的表达水平;采用qRT-PCR和Western blot法检测转染后Cx43的mRNA及蛋白表达水平的变化。利用CCK-8法检测细胞增殖能力,Transwell小室法检测细胞迁移和侵袭能力。结果 MCF-7和MDA-MB-231中miR-206的表达均明显低于正常细胞株;其中MCF-7细胞中miR-206表达水平较MDAMB-231中低,Cx43表达水平较MDA-MB-231中高。转染慢病毒包装靶向增强miR-206表达的shRNA载体LV-hsa-miR-206(1569-11)后,MCF-7细胞的miR-206表达明显上升,Cx43的mRNA表达水平及蛋白表达水平均明显下降;细胞的增殖、迁移和侵袭能力明显降低。转染慢病毒包装靶向抑制miR-206表达的shRNA载体LV-hsa-miR-206-inhibition后,MDAMB-231细胞的miR-206表达明显下降,Cx43 mRNA表达水平及蛋白表达水平均明显上升;细胞的增殖、侵袭和迁移能力明显增强;以上差异均具有统计学意义(P<0.05)。结论干预miR-206的表达可导致Cx43表达水平的变化,并影响人乳腺癌细胞MCF-7、MDA-MB-231的增殖、迁移和侵袭能力;miR-206可能通过抑制Cx43的表达调节了乳腺癌细胞的转移能力。 Objective To explore the roles of microRNA-206 （miR-206） and connexin 43 （ the cell proliferation, migration and invasion of breast cancer. Methods With qRT-PCR, the expression levels of breast cancer cell lines MDA-MB-231 and MCF-7 and normal mammary gland MCF-10A were detected. The levels of miR-206 in MDA-MB-231 and MCF-7 cells were up-regulated regulated by lentiviral vector-mediated transfection. blotting. Cell migratory and invasive abilities were Cx43 expression was detected by qRT-PCR and measured by Transwell chamber assay. Results Cx43 ） in miR-206 cell line or down- Western It was found that there was obviously lower expression of miR-206 in breast cancer cells than in normal cells. MCF-7 cells showed lower miR-206 level but higher Cx43 level than MDA-MB-231 cells. After shRNA-mediated miR- 206 up-regulation, MCF-7 cells displayed increase of miR-206 level, decrease of Cx43 mRNA and protein expression, and suppression of cell proliferation, migration and invasion （ P 〈 0. 05 ）. After shRNA-mediated miR-206 down-regulation, MDA-MB-231 cells displayed decrease of miR-206 level, increase of Cx43 mRNA and protein expression, and enhancement of cell proliferation, migration and invasion （P 〈 0. 05）. CoNclusion MicroRNA-206 inhibits migration and invasion of breast cancer cells through down-regulation of Cx43 expres- sion possibly, which indicates the tumor-suppressive function of microRNA-206 in breast cancer.
作者 杜俊泽 范林军 齐晓伟 孙鹏 王明浩 明佳 姜军
机构地区 第三军医大学西南医院乳腺病中心 重庆医科大学附属第二医院三腺外科
出处 《第三军医大学学报》 CAS CSCD 北大核心 2014年第2期115-120,共6页 Journal of Third Military Medical University
基金 国家自然科学基金(81172539) 重庆市自然科学基金(2011jjA0695)~~
关键词 乳腺肿瘤 肿瘤细胞 培养的 microRNA-206 连接蛋白43 细胞运动 肿瘤侵袭 breast neoplasms tumor cells, cultured microRNA-206 connexin 43 cell movement neoplasm invasiveness
分类号 R394.3 [医药卫生—医学遗传学] R73-37 [医药卫生—肿瘤]
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参考文献17

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共引文献8

同被引文献54

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引证文献6

二级引证文献16

第三军医大学学报
2014年 第2期

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