摘要
目的 :探讨ATP对不死化人成纤维细胞增殖及其细胞膜蛋白表达的影响。方法 :将正常人TIG- 7和OUMS - 36细胞株 ,不死化人KMST - 6和SUSM - 1细胞株在不同浓度的ATP ,ADP ,AMP条件下分别进行2 4和 96h的常规细胞培养 ,观察存活细胞数目 ,DNA的合成和 [3 2 P]ATP标记膜蛋白的表达。结果 :培养 96h后 ,0 4mmol/LATP对不死化细胞KMST - 6的抑制率为 77%且DNA合成明显地被抑制 ,而正常OUMS - 36细胞抑制率为 41%且DNA合成无明显改变。在 1mmol/LATP时 ,多数KMST - 6细胞发生死亡 ,而正常OUMS - 36细胞的增殖无明显影响。当ADP ,AMP和腺苷或磷酸处理的细胞 ,仅有ADP处理的不死化细胞存活数目减少 (P <0 0 1)。与正常细胞比较 ,不死化细胞 30kD、31kD、33kD和 40kD的 [3 2 P]-ATP标记膜蛋白呈高表达。结论 :ATP对不死化人成纤维细胞的增殖有明显的抑制作用 ,并且使磷酸化细胞膜蛋白表达增高。
AIM: To investigate the effect of ATP on proliferation and membrane protein expression in immortalized human fibroblasts. METHODS: Applying the normal human fibroblast cell line TIG-7 and OUMS-36, the immortalized human fibroblast cell line KMST-6 and SUSM-1 to culture for 24 hours and 96 hours with the different concentrations of ATP,ADP and AMP, respectively. The number of cell alive, DNA synthesis and the expression of [ 32P]-ATP labeled proteins in the membrane fraction were also observed. RESULTS: After 96 hours culture, the proliferation inhibition in immortalized cells exposed to 0.4mmol/L ATP was 77%, while that of normal OUMS-36 cells treated with the same concentration of ATP was 41%; the DNA synthesis of the immortalized cells exposed to 0.4mmol/L ATP was greater suppressed than that of normal OUMS-36 cells. ADP was the only reagent tested that reduced the number of cells significantly(P<0.01).When the immortalized cells exposed to ADP,AMP, adenosine, and phosphoric acid. In the presence of 1 mmol/L ATP, many KMST-6 cells died, but normal OUMS-36 cells did not. Compared with normal cells, the expresson of [ 32P]-ATP labeled 30 kD, 31 kD, 33 kD and 40 kD proteins were markedly higher in the immortalized cells. CONCLUSION: ATP inhibited the proliferation and increased membrane protein expression in immortalized human fibroblasts.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2000年第12期1270-1273,共4页
Chinese Journal of Pathophysiology
