摘要
目的：探讨p21和增殖细胞核抗原（proliferating cell nuclear antigen，PCNA）的相互关系及它们在肝硬化、肝细胞不典型增生（LCD）、肝细胞癌(HCC)形成中的作用。 方法：运用S-P法对60例单纯肝硬化、30例癌旁肝硬化及27例HCC的p21、PCNA及HBsAg表达情况进行实验研究。 结果：癌旁肝硬化、单纯肝硬化、癌组织p21阳性率分别为90%、68.33%、62.96%。癌旁肝硬化与单纯肝硬化及癌组织之间，差别有显著性意义(P＜0.05＝。伴LCD改变的肝硬化，p21、PCNA及HBsAg阳性率分别为92.45%、73.59%、64.92%均高于不伴LCD改变的肝硬化组织（P＜0.05＝。HBsAg阳性及PCNA表达阳性的肝硬化组织，其p21阳性表达率分别为87.32%、86.21%，均明显高于HBsAg阴性及PCNA阴性的肝硬化组织（P＜0.01＝。 结论：(1)p21的过度表达可能与HCC的起始过程有关，在HCC形成发展的早期阶段发挥重要作用。(2)LCD是一群具有肿瘤性增殖潜能的癌前细胞群，尤其是伴有HBV感染，且有p21及PCNA共同过量表达者，有发生HCC的高度危险。(3)HBV感染及PCNA过量表达与p21表达密切相关。
Objective: To probe the role of ras oncogene p21 product, proliferating cell nuclear antigen (PCNA) and hepatitis B virus infection in the development of cirrhotic nodules, liver cell dysplasia and hepatocellular carcinoma (HCC). Methods: S-P immunohistochemical technique was used to analyze the expression of p21, PCNA and HBsAg was studied in 30 cases of cirrhosis with HCC and 60 cases without HCC and 27 HCC cases. Results: The positive rates for p21 in cirrhosis with or without HCC, and HCC tissue were 90%, 68.33%, and 62.96%, respectively. The positive rates for p21, PCNA and HBsAg in cirrhotic nodules with LCD were 92.45%, 73.59% and 64.92%, respectively, significantly higher than those without LCD (P<0.05). The incidence of p21 expression in hepatocytes was significantly higher in HBsAg- and PCNA positive cases than in negative ones (P<0.01). Conclusion: (1)The overexpression of p21 may play an important role in the early stage of the development of HCC; (2)LCD are precancerous cells which have the ability to initiate neoplastic growth and present a high risk of development of HCC especially combining HBV infection and the overexpression of p21 and PCNA. (3)The expression level of p21 in liver cirrhosis is related to HBV infection and the expression level of PCNA.
出处
《中华肝脏病杂志》
CAS
CSCD
2000年第6期343-345,共3页
Chinese Journal of Hepatology
关键词
肝硬化
p2基因
PCNA
肝细胞不典型增生
Oncogene protein p21(ras)
Proliferating cell nuclear antigen
Hepatitis B virus
Liver cirrhosis
Carcinoma,hepatocellular
