摘要
为明确 Rubisco活化酶在叶片衰老期间是否对 Rubisco羧化活性和光合速率起调节作用 ,本文研究了水稻剑叶衰老过程中叶片光合速率、可溶性蛋白含量、Rubisco初始羧化活力及含量 ,Rubisco活化酶活力及含量 .结果表明 :净光合速率与 Rubisco初始羧化活力和 Rubisco活化酶的活力间分别为 r2=0 .9663和 r2 =0 .70 2 ,Rubisco初始羧化活力与 Rubisco活化酶的活力和含量间分别为 r2 =0 .81 0 3和r2 =0 .81 4 3 ,Rubisco活化酶含量与 Rubisco和可溶性蛋白间分别为 r2 =0 .92 57和 0 .8675,Rubisco活化酶的活力与含量间 r2 =0 .7587.Rubisco活化酶的含量占 Rubisco含量的 0 .5%～ 1 .0 % ,占叶片可溶性总蛋白质的 0 .5%～ 0 .6% ,随着剑叶衰老 Rubisco活化酶所占的比值加速下降 .这表明水稻剑叶衰老期间 ,Rubisco活化酶对维持 Rubisco初始羧化活力和净光合速率有重要调节作用 .
Photosynthetic rate , contents of soluble protein, chlorophyll, Rubisco and Rubisco activase, activities of Rubisco initial carboxylation and Rubisco activase were traced to clarify whether Rubisco activase regulates Rubisco activity and photosynthetic rate during flag leaf senescence. There were highly positive correlation between Rubisco activase activity and Rubisco initial carboxylation activity ( r 2=0. 810 3 ), and between Rubisco activase activity and photosynthetic rate (r 2=0.702), between Rubisco activase content and Rubisco activase activity ( r 2=0.814 3) as well as between Rubisco initial carboxylation activity and photosynthetic rate ( r 2= 0.966 3 ). The ratio of Rubisco activase to Rubisco content (0.5% 1.0%) or soluble protein content (0.5% 0.6%) was higher in young leaf than in old leaf. It can be suggested that Rubisco activase play an important role in controlling Rubisco initial activity and Pn in process of leaf senescence in rice plant.[WT5”BZ] [WT5”HZ]
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2000年第2期119-124,共6页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
The National Natural Science Foundation!(395 70 432 )
