摘要
目的:考察影响酶提取菠萝皮色素的主要因素,并确定酶辅助提取菠萝皮色素的最佳工艺条件。方法:采用单因素实验以色素浸提值为指标,考察了浸提时间、浸提温度、乙醇浓度、料液比对菠萝皮色素提取的影响,通过正交试验得到最佳的提取工艺。结果:酶辅助提取菠萝皮色素的最佳工艺条件是:乙醇浓度70%,提取温度50℃,提取时间80 min,料液比1∶50。结论:酶辅助提取法具有提取率高、速度快,溶剂用量少、安全、节能、设备简单等优点。本实验为优化菠萝皮色素的提取工艺奠定了基础。
Objective ：To discover main affecting factors and optimize extraction process of pineapple peel pigment by en- zyme auxiliary method. Method：Single-factor experiment was adopted and content of pigment from pineapple peel was taken as index.The extraction time, extraction temperature, ethanol concentration and the ratio of solid to liquid were se- lected as variable factors. Result：The optimum process conditions of pineapple peel pigment were as follows：70% ethanol for 1：50 ratio of solid to liquid in the process of extraction, extracting for 80 min at 50 ℃. Conclusion ： The advantages of enzyme auxiliary method are high extraction rate ,fast speed, less solvent consumption, safety, energy saving, simple equip- ment, etc. This experiment provide reference for optimizing extraction process of pineapple peel pigment.
出处
《山西中医学院学报》
2013年第5期37-39,共3页
Journal of Shanxi College of Traditional Chinese Medicine
基金
浙江中医药大学生物工程学院学生科研基金项目(201208)
关键词
酶辅助提取
菠萝皮色素
正交试验
enzyme auxiliary extraction
pineapple peel pigment
orthogonal design
