摘要
以籼稻七山占（Qishanzhan）和粳稻秋光（Akihikari）杂交衍生的重组自交系群体为材料，对穗长、穗重、颖花数等11个穗部性状进行数量性状基因定位（quantitative trait loci, QTL）分析。采用完备区间作图法，共检测到14个控制穗部性状的QTL，包括2个穗长 QTL，1 个穗重QTL，2 个一次枝梗数QTL，2 个二次枝梗数QTL，2 个颖花数QTL，3 个结实率QTL，1 个千粒重QTL 和1 个着粒密度 QTL，它们分布于第1，3，4，5，6，7，11，12号染色体上。进一步分析发现，控制穗部性状的QTL多分布在第1号染色体上，数量占总数的 40%以上，表明1号染色体相关基因的表达情况将直接影响水稻产量。控制穗部性状的单个QTL的贡献率在8.06%-58.62%之间，其中有6个增效等位基因来源于七山占，8个来源于秋光，这些结果将为水稻穗部性状遗传改良提供理论基础。
Recombinant inbred lines （RILs） derived from a cross between Akihikari （japonica） /Qishanzhan（indica） were used to map QTL controlling panicle traits （panicle length, panicle weight, spikelets number per panicle and so on）. Based on the Inclusive Composite Interval Mapping （ICIM）, a total of 14 QTLs were detected, containing 2 QTLs for PL 1 QTL for PW, 2 QTLs for PBN, 2 QTLs for SBN, 2 QTLs for SNP, 3 QTLs for SSR, 1 QTL for GD and 1 QTL for TGW, which were identified on chromosomes 1,3,4,5,6,7,11 and 12. Further analysis showed that the number of QTLs which detected on chromosome 1 accounted for more than 40% of the totals, which implied that the expression of gene on chromosome 1 played an important role in rice panicle traits. Furthermore, the contribution of single QTL in this study was between 8.06%-58.62%, and there were 8 efficient alleles from the typical japonica rice ＂Akihilari＂ and 6 efficient alleles from indica rice ＂Qishanzhan＇. All of these will be the basis of molecular genetic improvement for rice panicle traits.
出处
《沈阳农业大学学报》
CAS
CSCD
北大核心
2013年第6期727-732,共6页
Journal of Shenyang Agricultural University
基金
辽宁省教育厅重点实验室项目(LS2010147)
