摘要
目的：探讨虎潜丸治疗骨质疏松症的作用机制.方法：雌性SD大鼠40只,体质量180～220 g,中位数200 g,随机分为虎潜丸组、雌激素组、模型组、假手术组,每组10只.虎潜丸组、雌激素组、模型组切除双侧卵巢造模.7 d后,虎潜丸组予以虎潜丸水煎液灌胃,雌激素组予以尼尔雌醇混悬液灌胃,模型组、假手术组予以蒸馏水灌胃;每100 g体质量0.875 mL,每日灌胃1次,连续干预12周.比较各组大鼠椎骨骨密度及椎骨、肾组织中转化生长因子β1的表达.结果：虎潜丸组和雌激素组各有1只大鼠死亡,共纳入研究38只.干预12周后,各组大鼠椎骨骨密度值比较,差异有统计学意义[（0.210±0.028）g·cm-2,（0.205±0.030）g·cm-2,（0.171±0.036）g·cm-2,（0.205±0.034）g·cm-2;F=7.561,P=0.001];虎潜丸组、雌激素组、假手术组均高于模型组（P=0.002,P=0.002,P=0.013）;虎潜丸组与雌激素组、假手术组相比,差异均无统计学意义（P=0.459,P=0.224）;雌激素组与假手术组相比,差异无统计学意义（P=0.231）.各组大鼠椎骨转化生长因子β1灰度值比较,差异无统计学意义（169.060±5.545,167.420±6.238,162.400±3.882,168.130±7.899;F=2.176,P=0.109）.各组大鼠肾组织转化生长因子β1光密度值比较,差异有统计学意义（172.600±5.019,158.200±8.038,155.130±4.542,157.570±5.524;F=15.568,P=0.000）;虎潜丸组高于雌激素组、模型组和假手术组（P=0.000;P=0.000;P=0.000）;雌激素组与模型组、假手术组比较,差异均无统计学意义（P=0.478,P=0.490）;假手术组高于模型组（P=0.038）.结论：虎潜丸可增加大鼠腰椎椎骨骨密度,上调肾组织中转化生长因子β1的表达,可能是其治疗骨质疏松症的机制之一.
Objective：To explore the mechanisms of action of HUQIAN PILL in the treatment of osteoporosis. Methods：Forty female SD rats,ranged in weight from 180 to 220 g（ Median = 200 g） , were randomly divided into HUQIAN PILL group, estrogen group, model group and sham-operation group, 10 rats in each group. The rats models of osteoporosis were created by ovariectomy in HUQIAN PILL group, estrogen group and model group. Seven days after operation,rats were intragastric administrated with HUQIAN PILL decoction, nilest- riol suspension and distilled water（0. 875 mL/100 g）respectively,one time daily for 12 consecutive weeks. The bone density of vertebrae and transforming growth factor betal （ TGFβ1 ） expression in vertebra and nephridial tissue were detected and compared among the 4 groups. Results：One rat died in HUQIAN PILL group and one rat died in estrogen group,so 38 rats were included in the study. After 12 weeks of drug intervention, there were statistical differences in the bone density values of rat vertebra among the 4 groups （0. 210 ＋/-0. 028,0. 205 ＋/- 0. 030,0.171 ＋/- 0.036,0. 205 ＋/- 0. 034 g/cm （ 2 ）, F = 7.561, P = 0.001 ）. The bone density values of HUQIAN PILL group, estro- gen group and sham-operation group were all higher than that of model group （ P = 0. 002, P = 0. 002, P = 0.013 ）. The HUQIAN PILL group was similar to estrogen group and sham-operation group in the bone density values （ P = 0. 459, P = 0. 224 ）, and there were no statistical differences between estrogen group and sham-operation group （ P = 0. 231 ）. There were no statistical differences in the gray values of TGF-β1 in vertebra among the 4 groups（ 169.060 ＋/- 5. 545,167. 420 ＋/- 6. 238,162. 400 ＋/- 3. 882,168. 130 ＋/- 7. 899, F = 2. 176, P = 0. 109）. There were statistical differences in the optical density （OD） values of TGF- β1 in nephridial tissue among the 4 groups （ 172. 600 ＋/-5.019,158. 200 ＋/-8. 038,155.130 ＋/-4. 542,157. 570 ＋/-5. 524, F = 15. 568, P =0. 000）. The OD value of TGF-β1 of HUQIAN PILL group was higher than that of estrogen group, model group and sham-operation group respectively （P = 0. 000, P = 0. 000, P = 0. 000）. Estrogen group was similar to model group and sham-operation group （ P = 0. 478,P =0.490 ） in the OD value of TGF- β1, and the sham-operation group surpassed the model group（P = 0.038 ）. Conclusion：The bone density of lumbar vertebra can be increased and the expression of TGF-β1 in nephridial tissue can be up-regulated in rat model by using HUQIAN PILL,and it may be one of the mecha- nisms of action for HUQIAN PILL in the treatment of osteoporosis.
出处
《中医正骨》
2013年第12期11-14,共4页
The Journal of Traditional Chinese Orthopedics and Traumatology
基金
湖南省科技厅科研基金项目(2011SK3106)
关键词
骨质疏松
骨密度
转化生长因子Β1
动物实验
大鼠
虎潜丸
Osteoporosis
Bone density
Transforming growth factor betal
Animal experimentation
Rats ： HUOIAN PILL
