摘要
目的:研究碱性成纤维细胞生长因子(bFGF)抑制放射性诱导小鼠C17.2神经干细胞凋亡的作用。方法:用直线加速器进行总剂量为8 Gy外照射C17.2神经干细胞建立放射损伤细胞模型,将处理后的C17.2神经干细胞悬液以1×108/L的浓度接种于96孔板中,每孔加细胞悬液200μl,然后分别加入bFGF 0(对照),25,50和100μg/L干预24 h处理。用4-甲基偶氮唑盐法检测细胞活性并拟合生存曲线,用流式细胞仪检测细胞凋亡率。结果:随bFGF浓度增加,放射诱导后的C17.2神经干细胞增殖比明显增加(P<0.01),呈现明显剂量-效应关系(r=0.628,P<0.01)。在一定放射剂量下,随着bFGF浓度增加C17.2神经干细胞生存曲线右移,表明bFGF能够提高神经干细胞的放射耐受性(r=0.569,P<0.01)。bFGF为100μg/L时,C17.2神经干细胞活性最强,且无细胞毒性作用。流式细胞仪测定的对照组C17.2神经干细胞凋亡率明显高于bFGF 25和50μg/L(P<0.01)处理组,并且凋亡率在bFGF 25,50和100μg/L组间也存在显著差异(P<0.01)。结论:bFGF能显著抑制放射诱导的小鼠C17.2神经干细胞凋亡,其机制有待进一步研究。
Objective： To study the effect of basic fibroblast growth factor （bFGF） on the radiation induced apoptosis of mouse C17.2 neural stem cells. Methods： Linear irradiation accelerator （total dose = 8Gy） was used to induce C17.2 neural stem apoptosis, and the radiated cells were seeded in 96 well plates with 1 × 10^8/L concentration at the volume of 200 μl. Then bFGF were added at the concentration of 0 （control）, 25, 50 or 100 μg/L at 24 hours, respectively. The cell viability was measured by 4-methyl thiazolyl tetrazolium and survival curves were drawn, and the rate of cell apoptosis was determined by flow cell cytometry （FCC）. Results： With the increasing of bFGF concentration, C17.2 neural stem cell proliferation rate was significantly increased （ P 〈 0.01 ） in a dose-dependent manner （ r = 0. 628, P 〈 0. 01）. The survival curve of radiated C17.2 neural stem cell was right-shifted along with the increase of bFGF concentrations, sugges- ting that bFGF increase the radiation tolerance （r = 0.569, P 〈 0.01 ）. The highest cell activity without cell toxicity was observed under the bFGF treatment at 100 μg/L. FCC revealed a decreased cell apoptosis after treatment with bFGF at 25or 50 μg/L （ P 〈 0.01 ）. Significance was also observed for the cell apoptosis among the groups treated with bFGF at 25, 50 and 100 μg/L （P 〈 0.01 ）, respectively. Conclusion： Exogenous bFGF significantly inhibited the radiation induced apoptosis of C17.2 neural stem cells and the underlying mechanism needs to be studied further.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2014年第1期98-102,共5页
Chinese Journal of Neuroanatomy
