摘要
目的：观察KCl刺激培养的大鼠E17脑皮质神经细胞后不同时间点Dlx2、Arx、Rbl基因的表达，探讨Dlx2、Arx、Rbl的表达的变化。方法：原代细胞培养获取胎龄E17大鼠脑皮质神经细胞，采用反转录．聚合酶链反应（reverse transcription polymerase chain reaction，RT-PCR）技术检测Dlx2、Arx、Rbl在经KCl刺激0．5～24h后的表达变化情况。结果：RT—PCR结果显示KCl诱导组不同时间点神经细胞中RblmRNA表达在0．5～6h时呈现递增的趋势，而在12hmRNA表达明显大幅度降低，24h又出现上升；Dlx2随着KCl的诱导表现出表达的一个长时程增强的过程，其mRNA表达随着时间的变化而增多，并在24h达到表达的高峰；ArxmRNA的表达则出现增强趋势，持续24h，但此时略有下降。结论：经KCl刺激后Dlx2mRNA表达升高对胚胎期神经细胞的存活功能可能具有重要作用。
Objective： To observe the expressions of Dlx2, Arx, Rbl genes after KC1 stimulation of cultured El7 cortex neurons at different time points, and to investigate the characteristics and the change of Dlx2, Arx, Rbl expression on transcription level. Methods： The method of primary cell culture was used for El7 rat cerebral cortical neuron, mRNA was analyzed by using reverse transcription - polymerase chain reaction （RT-PCR） to detect the expressions of Dlx2, Arx, Rbl fbllowing KCI stimulation at the time points from 0.5 to 24 h. Results： RT-PCR results showed that there was an increasing trend of Rbl mRNA in KC1 induced group through 0.5-6 h, while at 12 h it was significantly reduced and it was upward again at 24 h; With a long-term potentiation in the process, the expression of Dlx2 increased and peaked at 24 h; An Arx mRNA increasing trend persisted for 24 h and here after, the expression even slightly decreased. Conclu- sion： The data showed that increased expression of Dlx2 by KC1 stimulation might play an important role in embryonic neuron survival.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2014年第1期81-85,共5页
Chinese Journal of Neuroanatomy
基金
国家自然科学基金(81360373)
