摘要
目的:观察单纯性脑震荡(pure cerebral concussion,PCC)大鼠前额叶皮质(prefrontal cortex,PC)、颞叶皮质(temporal cortex,TC)和梨状皮质(piriform cortex,Pir)内小胶质细胞(microglia,MG)的反应和时程变化规律,探讨小胶质细胞与脑损伤之间的病理联系。方法:采用自制金属单摆闭合式脑损伤打击装置制备清醒状态下PCC模型,随机分为伤后3 h、12 h、1 d、2 d、3 d、7 d六个损伤组(n=5),另设正常对照组(n=5)。采用OX-42单克隆抗体(MG特异性标记物)进行免疫组织化学和免疫荧光染色,观察PCC组和正常对照组大鼠PC、TC和Pir中OX-42的表达变化。结果:正常对照组大鼠PC、TC和Pir内OX-42免疫阳性产物的表达很弱,OX-42免疫阳性的小胶质细胞的数量少而轮廓不清。损伤后OX-42免疫阳性产物的表达和阳性细胞的数量逐渐增加,3 d时达高峰,7 d后有下降趋势,但仍高于正常组(P<0.05)。结论:PCC损伤早期PC、TC和Pir中MG出现激活的形态学变化,提示MG可能参与了PCC致伤后的病理变化。
Objective： To study the response and change of the microglia（MG） in the prefrontal cortex（ PC）, temporal cortex（TC） and piriform cortex（Pir） after the pure cerebral concussion（PCC） and to explore the relationship between mi- croglia and pathological changes after brain injury. Methods： Under the waking condition, the PCC models of the rats were established by using a metallic pendulum-striker concussive device. The rats were randomly divided into 3 h, 12 h, 1 d, 2 d, 3 d and 7 d（n = 5）groups after PCC injury. One normal group（n =5） was used as the control. The distribution and changes of OX-42 expression in the PC, TC and Pir between the PCC and the control groups were observed by the im- munohistochemical and immunofluorescence histochemical staining. Results： The expression of OX-42-immunoreaetivity was weak and the profile of a small number of the positive cells was unclear in the control group. The expression of OX- 42-immunoreaetivity and the number of the OX-42-imnlunopositive cells increased gradually from 3 h to 3 d in the PC ,TC and Pir after brain injury when compared with the control group, and were strongest at 3 d and showed a downward but still higher than the control group until 7 d（ P 〈 0.05 ）. Conclusion： That microglia was activated with the changes in mor- phology and number in the PC ,TC and Pir during the early stage of the brain injury suggest that MG may be involved in the pathological changes after PCC.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2014年第1期29-34,共6页
Chinese Journal of Neuroanatomy
基金
国家自然科学基金(31100769
30560041)
云南省自然科学基金(2008CD052)
