摘要
目的:探讨脑卒中后抑郁(PSD)大鼠海马脑源性神经营养因子(BDNF)及其高亲和力受体酪氨酸激酶B(TrkB)蛋白的表达变化。方法:24只雌性SD大鼠随机分为正常组、抑郁组、脑卒中组及PSD组,每组6只。采用线栓法建立局灶性脑缺血(卒中)模型;采用孤养方法建立抑郁模型;局灶性脑缺血模型加以慢性不可预见的中等应激刺激和孤养法建立PSD模型;并设正常组做为正常对照。应用免疫组化检测各组大鼠造模后第29天大鼠海马BDNF和TrkB免疫阳性细胞数的表达变化。结果:PSD组海马BDNF免疫阳性细胞数最少[(8.56±1.67)个/视野]。PSD组、抑郁组及脑卒中组与正常对照组相比BDNF阳性细胞数均明显减少(P<0.05);PSD组海马TrkB免疫阳性细胞数最少[(6.44±1.13)个/视野],与各对照组相比,差异均有统计学意义(P<0.05)。结论:PSD大鼠海马BDNF及其高亲和力受体TrkB蛋白表达减少可能在PSD发病过程中发挥了一定的作用。
Objective： To explore the expression of BDNF and high-affinity receptors TrkB protein in hippocampus of the post stroke depression in the rats. Methods： Focal cerebral ischemic rat models were made with thread embolization method. Post stroke depression rat models were established with comprehensive separately breeding and chronic unpredict- ed mild stress （CUMS） on this basis. Normal control group,depression group and stroke group were used to compare with PSD group. 6 rats were used in each group. Immunohistochemistry for detecting the expression of BDNF and TrkB in the hippocampus was used at 29th day since the CUMS. Results：The results showed that the number of BNDF immunoposi- tive cells in PSD group was the least than other groups [ （8.56 ± 1.67）, per microscope field of vision 1- Compared to control group, the number of BDNF immunopositive cells decreased significantly in the PSD group, depression group and stroke group（P 〈0.05）. The number of TrkB immunopositive cells in the hippocampus was least in PSD group than the other groups （ P 〈 0.05 ）. Conclusion ： The downregulation of BDNF and TrkB expression in the hippocampus may be re- sponsible for the pathogenesis of PSD.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2014年第1期19-22,共4页
Chinese Journal of Neuroanatomy
基金
云南省科技厅科研基金项目(2009 ZC126M)
