摘要
根据猪附红细胞体3-磷酸甘油醛脱氢酶样蛋白1基因（msgl）序列设计引物，对上海地区分离的小附红细胞体阳性猪全血DNA进行PCR扩增，获得小附红细胞体3-磷酸甘油醛脱氢酶样蛋白1基因（mpgl）并进行测序和分析，研究其遗传变异特点。结果显示，共获得了11条小附红细胞体mpgl基因序列，序列长度均为1011bp。同源性分析显示，所获得的1l条小附红细胞体mpgl基因序列之间的相似性为96．8％～99．9％，预测氨基酸序列的相似性在97．9％～100％之间；与GenBank中公布的猪附红细胞体msgl基因核苷酸序列相似性在96．6％～98．2％之间，氨基酸序列的相似性为98．2％～99．1％。系统进化分析表明，msgl基因与mpgl基因共同构成每个聚簇。
Mycoplasma parvum glyceraldehyde-3-phosphate dehydrogenase-like protein 1 gene（mpgl） was amplified from genomie DNAs of the blood of pigs infected with M. parvurn by PCR with a pair of primers designed based on the sequence of msgl. The PCR products were inserted into the vector pMD18- T. The recombinant plasmids were transformed into Escherichia coli DHSa and sequenced. The sequences of rnpgl gene and their genetic variation characteristics were analyzed. In result, 11 rnpgl genes were se- quenced successfully and all the nucleotide sequences were 1 011 bp in length. Homology analysis showed that the similarity of 11 mpgl gene, mpgl and msgl gene downloaded from GenBank was 96. 8% to 99.9%,and 96.6%//o to 98.2~,and 97.9% to 100% for nucleotide sequences,respectively,and 98.2% to 99.1~ for deduced amino acid sequences,respectively. The phylogenetic analysis revealed that all clusters were constructed with rnsgl and mpgl genes.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2014年第1期7-13,共7页
Chinese Veterinary Science
基金
公益性行业(农业)科研专项项目(200903036-05)
