摘要
目的于普通食管支架上口捆绑^125I粒子，通过动物实验探索其预防食管良性再狭窄的可行性及效果。方法于普通食管支架上口均匀捆绑8颗^125I粒子，制成预防食管良性再狭窄支架并进行动物实验。取32只比格犬，分为对照组、实验组，其中对照组（16只）置人捆绑空粒子的支架；实验组（16只）置入捆绑^125I粒子（33．3MBq×8）的支架。术后1、2、4、8周分别取各组犬4只，处死前行相关影像学检查及胃镜检查；处死后取各组支架上端2cm范围内的食管组织行病理学等检查。结果所有食管支架均释放到位，ECT复查未发现放射源泄漏。测量支架上口管腔内径，结果显示随着时间延长管腔逐渐变窄，4、8周时对照组较实验组更为狭窄，差异有统计学意义（P〈0．05）；免疫组化结果示平均光密度值4周时实验组较对照组减低，差异有统计学意义（P〈0．05）；羟脯氨酸及总氨基酸含量随时间延长逐渐上升并且术后4、8周时实验组较对照组含量减低，差异有统计学意义（P〈0．05）。实验观察期间内实验组支架周围的气管与大血管等与对照组肉眼、光镜所见基本一致。结论该新型支架用于食管良性狭窄的预防是可行的、安全的，并具有一定的预防和减轻支架术后良性再狭窄的效果。
Objective To investigate the feasibility and preventive effect of benign esophageal rest-enosis by binding ^125I seeds to upper esophageal stent through animal experiments. Methods Eight ^125I seeds were evenly bound to upper normal esophageal stents for the animal experiments. A total of 32 beagle dogs were randomly assigned to 2 groups： experimental group, implanted with esophageal stents with eight ^125I seeds （33.3 MBq）, and the control （stents without ^125I seeds）. Four dogs of each group were killed at the 1st, 2nd, 4th, and 8th week after imaging studies. The tissue of 2 cm upper stent underwent pathology analysis. Results All the novel stents were successfully implanted. No radioactive leak was detected by ECT. The lumen diameter of the top stent showed the tract gradually narrowed and at the 4th and 8th weeks, the experiment group narrowed more seriously compared with the control group and the difference was statisti-cally significant （P 〈 0. 05 ）. PCNA, α-SMA mean optical density were significantly different in the 4th week. Both hydroxyproline and total amino acid increased progressively, with significant difference at the 4th and 8th weeks. The macroscopic and optical findings of the trachea and major vessels were basically the same in both groups. Conclusion The novel stent is feasible and safe for preventing benign esophageal restenosis and preventing benign esophageal stent restenosis.
出处
《中华消化内镜杂志》
2014年第1期37-41,共5页
Chinese Journal of Digestive Endoscopy
基金
国家自然基金（No.81071238）
