摘要
目的 :构建结核杆菌HSP70重组真核表达质粒。方法 :用PCR方法从结核杆菌H37RV基因组中扩增出结核杆菌HSP70基因NDA序列 ,应用T A克隆技术 ,克隆到质粒GPEM Gvector,经DNA测序证实基因碱基无误后 ,亚克隆到真核表达质粒PCDNA3 1(+ ) ,构建成PCDNA3 1 HSP70重组质粒。结果 :经酶切鉴定 ,证实重组质粒构建正确。结论 :结核杆菌HSP70真核表达载体构建成功。
Objective:To construct a recombinant eukaryotic expression plasmid of mycobacterium tuberculosis HSP 70. Methods: The full length of TB.hsp70 gene from the genome of M.tuberculosis H37RV was amplified and cloned into pGEM-T vector with the T-A cloning technique.Then,the recombinant plasmid was digested with BamHⅠand EocRⅠ and subcloned into PCDNA31(+).Results By restriction enzyme digestion and DNA sequencing,it was confirmed that the recombinant eukaryotic expression plasmid of M.tuberculosis HSP 70 has been successfully constructed.
出处
《广州医学院学报》
2000年第4期9-12,共4页
Academic Journal of Guangzhou Medical College
