摘要
以提取过黄酮的淫羊藿药渣为原材料,热水法提取多糖,测定淫羊藿药渣多糖的总糖含量、糖醛酸含量和蛋白质含量,并对多糖结构进行红外光谱分析。通过体外抗氧化实验研究其对DPPH自由基、超氧阴离子和羟基自由基的清除能力。结果表明,淫羊藿药渣多糖的总糖质量分数为73.39%,糖醛酸质量分数为6.33%,蛋白质质量分数为1.87%,对DPPH自由基、超氧阴离子和羟基自由基的最大清除率分别为79.96%、92.77%和69.12%,其IC50值分别为0.26、0.03、0.76mg/mL。
Epimedium residue which was firstly conducted to extract flavonoid was applied to extract polysaccharide. In addition,the total contents of carbohydrate,uronic acid and protein of Epimedium residue polysaccharide were measured by chemical methods,and the structural characteristic of ERP was detected by FT-IR spectra. Furthermore,DPPH,superoxide anion radicals,hydroxyl radical scavenging abilities of ERP were analyzed for antioxidant activity in vitro. The results showed that the contents of carbohydrate,uronic acid and protein were 73.39%,6.33% and 1.87%,respectively. The maximum inhibition percentage of DPPH,superoxide anion radicals and hydroxyl radical of ERP were 79.96%,92.77% and 69.12%,respectively. The IC50 values of those antioxidant activities were 0.26,0.03,0.76mg/mL,respectively.
出处
《食品工业科技》
CAS
CSCD
北大核心
2014年第2期143-145,153,共4页
Science and Technology of Food Industry
基金
四川省科技厅应用基础项目(2008JY0094-2)
关键词
淫羊藿
药渣
多糖
抗氧化
Epimedium
residue
polysaccharides
antioxidant activity
