摘要
文章对罗非鱼鱼鳍胶原蛋白的提取进行研究。通过单因素试验研究鱼鳍胶原蛋白提取的不同因素影响作用,并通过正交试验确定胃蛋白酶酶法提取胶原蛋白的最佳工艺。研究结果表明,在酶解时间6h、底物浓度5%、酶量5 500U g、pH为1.80和温度为40℃的条件下,胃蛋白酶水解罗非鱼鱼鳍效果较好。正交试验表明,酶法提取反应主要因素影响的顺序为:反应时间>酶量>底物浓度。
The extraction of collagen protein from tilapia fin is studied. The single factor experiment was conducted to study the effects of dif- ferent factors on pepsin hydrolysis of tilapia, and the best hydrolysis technology of tilapia pepsin hydrolysis were determined by orthogonal test. The results showed that the better effect of pepsin hydrolysis is in the enzymolysis time 6h, substrate concentration 5%, the amount of enzyme 5500 U/g, pH 1.80, the temperature 40 ~C. Orthogonal test showed that the order of effect is： reaction time 〉 enzyme amount 〉 substrate concentration.
出处
《广东石油化工学院学报》
2013年第6期24-26,共3页
Journal of Guangdong University of Petrochemical Technology
关键词
罗非鱼鱼鳍
胃蛋白酶
胶原蛋白
tilapia fin
pepsin hydrolysis
collagen protein
