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后处理对缺血再灌注损伤鼠肺CD4^+ T细胞活性的影响 被引量：2

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摘要 目的:研究后处理对在体缺血再灌注损伤鼠肺CD4+T细胞活性的影响,并分析其可能的肺保护作用机制。方法:构建大鼠在体肺脏缺血再灌注损伤模型,30只SD大鼠随机分为假手术对照组(Sham组)、缺血再灌注组(IR组)和缺血后处理组(IPostC组),每组10只。Sham组开胸游离左肺门穿阻断带而不结扎持续180 min;IR组缺血60 min后再灌注120 min;IPostC组缺血60 min后给予3个周期的5 min灌注/5 min缺血后处理,再灌注90 min。留取左肺组织、左支气管肺泡灌洗液,分别用于测定髓过氧化物酶(MPO)、IL-17、IL-8及CD4+T细胞含量。留取小块肺组织测定肺湿/干重比(W/D)。结果:Sham组肺组织W/D、支气管肺泡灌洗液中IL-8、IL-17及MPO含量和肺组织中IL-8、IL-17及CD4+T细胞含量明显低于IR组和IPostC组(P<0.05);IPostC组肺组织W/D、支气管肺泡灌洗液中IL-8、IL-17及MPO含量和肺组织中IL-8、IL-17及CD4+T细胞含量明显低于IR组(P<0.05)。结论:缺血后处理能明显减轻大鼠肺缺血再灌注早期损伤,其机制可能与抑制CD4+T淋巴细胞活性从而减轻肺组织炎症损害有关。
作者 伍火志 张浩 张大勇 袁江 马明生 陈鑫
机构地区 合肥医学院第五附属医院心胸外科 山东省莱芜市人民医院普外科 山东省滨州市人民医院心胸外科
出处 《实用医学杂志》 CAS 北大核心 2014年第1期49-51,共3页 The Journal of Practical Medicine
基金 广东省医学科学技术研究基金资助项目(编号:A2012622)
关键词 再灌注损伤 急性肺损伤 后处理 CD4+T细胞 大鼠
分类号 R563.8 [医药卫生—呼吸系统]
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参考文献13

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共引文献9

同被引文献27

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引证文献2

二级引证文献9

实用医学杂志
2014年 第1期

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