摘要
目的探讨降钙素与1,25维生素D对牙周炎导致大鼠牙槽骨丧失的影响。方法雄性Wistar大鼠125只随机分为空白对照组(A组)、牙周炎对照组(B组)、降钙素治疗组(C组,降钙素溶于生理盐水,2μg/kg,皮下注射)、1,25维生素D治疗组(D组,维生素D溶于花生油中,2μg/kg,灌胃)、降钙素和1,25维生素D联合治疗组(E组),每组25只。用正畸结扎丝结扎上颌磨牙牙颈部加高糖软食的方法建立牙周炎大鼠模型。在第2、4、6和8周末各组处死5只大鼠,测量双侧上颌第一磨牙牙周探诊深度(PD),观察牙槽骨水平;第8周末各组采取10只大鼠腹部降主动脉血,用酶联免疫吸附试验法(ELISA)检测血清中的骨碱性磷酸酶(BALP)及骨钙素(OC)的浓度。结果 4周、6周、8周时E组大鼠的PD低于B、C、D组(P<0.05)。6周、8周时E组大鼠牙槽骨丧失水平低于B、C、D组(P<0.05)。第8周时E组BALP及OC浓度显著高于B、C、D组(P<0.05)。结论降钙素和1,25维生素D均能部分拮抗牙周炎导致的牙槽骨丧失,二者联合应用效果更佳。
Objective To evaluate the effects of calcitonin and 1, 25 vitamin D on the bone loss in experimental periodontitis in rats. Methods A total of 125 male Wistar rats were randomly allocated to five groups according to the treatments during 8 weeks： normal control （group A, n=25）, periodontitis（group B, n=25）, calcitonin （group C, prepared in sterile saline at 2 mg/L, and 2 p,g/kg was administered daily, s.c., n=25）, 1,25 vitamin D （group D, prepared in corn oil daily at a concentration of 2 mg/L, and 2 p,g/kg was administered daily, p.o. , n=25）, 1,25 vitamin D plus calcitonin （group E, n= 25）. The experimental model of periodontitis was induced by ligating floss around mandibular first molars with orthodontic wires in B, C, D and E groups. Five rats from each group were sacrificed, and the specimens were prepared at 2, 4, 6 and 8- week. The probing depth （PD） and alveolar bone level were observed. The serum bone-specific alkaline phosphatase （BALP） and osteocalcin （OC） were measured by enzyme-linked immunosorbent assay （ELISA） at 8-week in each group. Results The values of PD were significantly lower after 4, 6 and 8 weeks in E group than those of B, C and D groups （P 〈 0.05）. The alveolar bone loss was significantly lower after 6 and 8 weeks in group E compared with that of B, C and D groups （P 〈 0.05）. The serum levels of BALP and OC were significantly higher after 8 weeks in E group than those of B, C and D groups （P 〈 0.05）. Conclusion The present study suggests that 1, 25 vitamin D and calcitonin can partially inhibit the alveolar bone loss induced by periodontitis. Especially, the application of both is more effective than either drug treatment alone.
出处
《天津医药》
CAS
北大核心
2014年第1期68-70,共3页
Tianjin Medical Journal
