摘要
目的探讨五味子乙素(SchB)对长波紫外线(UVA)诱导永生化人角质形成细胞(HaCat细胞)损伤后的保护作用及其可能的作用机制。方法用5 J/cm2的UVA照射HaCat细胞,给予不同浓度的SchB(0.1、0.01、0.001、0.000 1μmol/L)处理UVA损伤后的HaCat细胞,检测细胞超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-Px)的活性,乳酸脱氢酶(LDH)及NO含量。结果 UVA照射HaCat细胞后,SOD、GSH-Px活性降低,LDH及NO含量升高,给予不同浓度的SchB均能显著提高SOD、GSH-Px活性,降低LDH及NO含量,提高HaCat细胞的存活率,尤其以0.001μmol/L SchB的保护作用最强。结论 0.001μmol/L SchB减轻UVA对HaCat细胞损伤的效果最佳。
Objective To explore the protective effect of schisandrin B （SchB） on the permanent damage of human keratinocytes （HaCat） induced by long wave ultraviolet （UVA）, and its possible mechanism thereof. Methods After HaCat was treated by 5 J/cm~ UVA, different concentrations of SchB （03, 0.01, 0.001 and 0.000 1 μmol/L） were used to treat HaCat cells. The levels of superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px） activity, lactate dehydrogenase （LDH） and NO content were detected. Results The levels of SOD and GSH-Px activity were decreased, and he levels of LDH and NO content were increased in HaCat cells after being treated by UVA. The different concentrations of SehB showed significant ef- fects on the increased levels of SOD, GSH-Px activity and decreased levels of LDH and NO, and improved the survival rate of HaCat cells. The 0.001 μmol/L SchB showed the strongest protective effect. Conclusion The 0.001 p^mol/L SchB showed the best effect on the damage of HaCat cells induce the UVA.
出处
《天津医药》
CAS
北大核心
2014年第1期5-6,共2页
Tianjin Medical Journal
基金
国家自然科学基金资助项目(项目编号:81072964)
